UFLC法同时测定护肝胶囊中五味子醇甲、五味子甲素、五味子乙素  

Simultaneous determination of schizandrol A,schisandrin A and schisandrin B in Hugan Capsules by UFLC

作　　者：张林林[1] 王永吉[2] 李航[1] 张辉[1] 孙佳明[1] 

机构地区：[1]长春中医药大学中医药与生物工程研发中心,吉林长春130117 [2]长春中医药大学第一附属医院内儿科,吉林长春130021

出　　处：《中成药》2013年第1期96-99,共4页Chinese Traditional Patent Medicine

摘　　要：目的建立UFLC同时测定护肝胶囊(柴胡、五味子等)中五味子醇甲、五味子甲素和五味子乙素的方法。方法色谱柱Agilent SB C18(100 mm×4.6 mm,1.8μm);流动相为乙腈-水梯度洗脱;体积流量0.4 mL/min;柱温40℃;检测波长250 nm。结果五味子醇甲、五味子甲素、五味子乙素进样量分别在0.029～0.584μg(r=0.999 9)、0.016～0.328μg(r=0.999 6)、0.02～0.40μg(r=0.999 1)范围内具有良好的线性关系。平均回收率分别为99.7%、98.5%、99.2%;RSD分别为1.1%、2.3%、1.6%(n=5)。结论本方法简便、准确、重复性好,可用于护肝胶囊的质量控制。AIM To establish a UFLC method for determining sehizandrol A, schisandrin A and schisandrin B in Hugan Capsules （Bupleuri Radix, Schisandrae chinensis Fructus, etc. ）. METHODS The Agilent SB C18 column （100 mm ×4.6mm, 1.8 μm） was used. The mobile phase consisted of acetonitrile-water in gradient elution mode with a volume flow rate of 0. 4 mL/min. The detection wavelength was set at 2150 nm. RESULTS There were good linear relationships in the ranges of 0. 029-0. 584μg （ r = 0. 999 9 ） , 0. 016-0. 328μg （ r = 0. 999 6） , 0.02-0.40 μg （ r = 0. 999 1 ） , respectively. The average recoveries were 99.7% , 98.5% , 99.2% and RSDs were 1.1% , 2.3% , 1.6% （n = 5） , respectively. CONCLUSION The method is efficient and accu- rate with a good reproducibility and can be used for the quality control of Hugan Capsules.

关 键 词：护肝胶囊 五味子醇甲 五味子甲素 五味子乙素 UFLC 

分 类 号：R927.2[医药卫生—药学]