三七与丹参不同洗脱部位对大鼠血管球囊损伤再狭窄的治疗作用研究  

Research on the Treatment of SD Rats Vascular Balloon Injury with Salvia and Panax Notoginseng Different Ethanol Extracts

作　　者：王云飞[1] 潘从泽[1] 高秀梅[1] 王升启[2] 

机构地区：[1]天津中医药大学,天津300193 [2]军事医学科学院放射与辐射研究所,北京100850

出　　处：《辽宁中医杂志》2013年第1期170-173,共4页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金(81073093);国家科技部"重大新药创制"子课题(2011ZX09201-201)

摘　　要：目的:探讨三七与丹参不同提取部位对SD大鼠左侧颈总动脉球囊损伤再狭窄的治疗作用。方法:SD大鼠,雄性,82只,分为正常组、模型组、假手术组、阿伐脱他汀钙片组(3 mg.kg-1.d-1)、复方丹参片组(300 mg.kg-1.d-1)、50%洗脱部位高中低剂量组(300、150、75 mg.kg-1.d-1)、80%洗脱部位高中低剂量组(300、150、75 mg.kg-1.d-1)、95%洗脱部位高中低洗脱部位(300、150、75 mg.kg-1.d-1)灌胃。用球囊导管损伤建立大鼠颈总动脉内膜增生模型,7天后,测其血清中SOD、NO、MDA水平,计算其内膜(NIA)与中膜(MA)面积比。结果:50%洗脱部位中剂量组、95%洗脱部位高剂量组SOD与模型组比较有显著性差异。结论:丹参与三七50%、95%洗脱部位150 mg.kg-1.d-1可以抑制SD大鼠颈总动脉球囊损伤增生。Objective ： To investigate the treatment of SD rats vascular balloon injury with Salvia and Panax Notoginseng differ- ent ethanol extracts. Methods ：84 SD rats were randomly divided into 14 groups：normal group, model group, sham operation group, Alpha statin calcium group （3 mg·kg-1·d-1 ）, Fufang Danshen tablet group （300 mg·kg-1·d-1 ） ,50% Elution position high intermediate low dose group （ 300 mg·kg-1·d-1 1,150 mg·kg-1·d-1 ,75mg·kg-1·d-1 ）, 80% Elution position high inter- mediate low dose group（300 mg·kg-1·d-1-2,150 mg·kg-1·d-1,75 mg·kg-1·d-1）,95% Elution position high intermediate low dose group（300 mg·kg-1·d-1 , 150mg·kg-1·d-1 ,75 mg·kg-1·d-1 ） ,with intragastric administration. The balloon catheter injury method was used to establish model of intimal hyperplasia of rats carotid artery. After seven days, the serum SOD, NO and MDA levels were measured. The endometrium （NIA） and the membrane （MA） area ratio were calculated, hnmunohisto- chemical detection of vascular injury in PCNA,VEGF and NF - Kb expressions were observed. Results ：50% Elution position in- termediate dose group and 95% Elution position high dose group SOD compared with model group have significant difference. Conclusion：Salvia and Panax Notoginseng 50% as well as 95 % elution position （ 150mg·kg-1·d-1 ）can inhibit the SD rat ca- rotid artery balloon injury hyperplasia.

关 键 词：血管球囊损伤 内膜增生 再狭窄 丹参 三七 

分 类 号：R285[医药卫生—中药学]