生物强化组合工艺处理河水的三维荧光及生物多样性分析  

Excitation-emission matrix fluorescence spectra and microbial diversity analysis of river water in biological enhanced integrated process

作　　者：申颖洁[1] 廖日红[1] 战楠[1] 刘操[1] 孟庆义[1] 

机构地区：[1]北京市水利科学研究所,北京100048

出　　处：《环境工程学报》2013年第1期125-131,共7页Chinese Journal of Environmental Engineering

基　　金：北京市重大科技专项(D07060601980802);北京市科技新星计划(2009B31);国家"水体污染控制与治理"科技重大专项(2009ZX07314-009)

摘　　要：通过对受污染地表水进行生物滤池-臭氧预氧化-生物活性炭滤池工艺处理,考察生物强化条件下该项工艺对河水中主要污染物的净化效果,并采用EEM光谱技术进行了溶解性有机物变化和去除规律分析,利用PCR-DGGE技术进行各单元中微生物多样性对比分析。结果表明,生物强化组合工艺系统出水水质主要指标已达到/接近地表水环境质量标准(GB 3838-2002)Ⅳ类限值,生物强化滤池填料中微生物多样性指数和物种数均高于其他工艺单元。受污染河水DOM中主要的荧光物质有类芳香族蛋白质(荧光峰A、B和E)及类腐殖酸(荧光峰C)及类富里酸(荧光峰D),其中,A峰、B峰与E峰的中心位置分别位于225/340 nm、275/336 nm和225/298 nm,各特征荧光峰强度发生明显改变表明,污水中溶解性有机物的含量随系统处理过程而变化。The biofilter-ozonation-BAC process was constructed and used to treat polluted surface water, and the main pollutants removal rates were studied on the application of bio-augmentation technique. Fluores- cence spectrum of dissolved organic matter in the river water was analyzed, moreover, PCR-DGGE technology was applied to analyze microbial diversity. It was known from research that, the main water indexes of outlet wa- ter for the bio-augmentated experiment could meet or be close to the Surface Water Environment Quality Standard GB 3838-2002 IV, both the microbial diversity index and the species numbers in bio-augmentation biofilter were higher than that in other units. The fluorescent components in the river water DOM were mainly aromatic protein （Peak A, Peak B and Peak E） , humic-like fluorophores （Peak C） and fulvic-like fluorophores （Peak D） , in which the aromatic protein fluorophores Peak A,Peak B and Peak E occurred at 225/340 nm,275/336 nm and 225/298 nm, respectively. The fluorophores intensity of the characteristic fluorescence peaks varied significantly indicating that the concentration of DOM varied with the wastewater treatment process.

关 键 词：生物强化 受污染河水 溶解性有机物 三维荧光指纹光谱 微生物多样性 

分 类 号：X522[环境科学与工程—环境工程]