葛根素对早期糖尿病肾病大鼠肾组织病理变化与BMP-7蛋白及mRNA表达的影响  

Effects of Puerarin on the Pathological Change and Protein and mRNA Expressions of BMP-7 in Renal Tissue of Rats with Early Diabetic Nephropathy

作　　者：刘圣[1,2] 张小力[1,2] 余娜[1,2] 陈象青[1] 唐丽琴[1] 

机构地区：[1]安徽医科大学附属省立医院,合肥230001 [2]安徽中医学院药学院,合肥230031

出　　处：《中国药房》2013年第3期196-200,共5页China Pharmacy

基　　金：安徽省科技计划资助项目(No.08020303074);安徽省卫生厅医学科研课题(No.09C219)

摘　　要：目的:研究葛根素(Pue)对早期糖尿病肾病(DN)大鼠肾组织病理变化与骨形态发生蛋白-7(BMP-7)及mRNA表达的影响。方法:以链脲佐菌素(STZ)腹腔注射复制早期DN大鼠模型。动物分为正常对照、模型、依那普利(1mg/kg)与Pue高、中、低剂量(200、100、50mg/kg)组,灌胃给药,每天1次,连续5周。末次给药后检测大鼠24h尿量、体质量、肾/体质量比值(KW/BW);生化指标检测空腹血糖(FBG)、24h尿量尿素氮(BUN)、血肌酐(Scr)、胰岛素(Ins)、24h尿蛋白(Upro)及24h尿微量白蛋白(UmAlb);光镜和电镜观察肾组织形态学和超微结构的改变;免疫组化法和逆转录聚合酶链反应(RT-PCR)检测肾组织BMP-7蛋白及mRNA的表达。结果:与模型组比较,Pue高、中、低剂量组大鼠FBG、KW/BW、BUN、Scr水平降低,体质量、Ins水平升高,BMP-7蛋白及mRNA表达增多(P<0.01或P<0.05),肾组织病理形态学异常与超微结构病变改善;Pue高、中剂量组24h尿量、24hUpro、24hUmAlb显著降低(P<0.01或P<0.05)。结论:Pue可显著升高早期DN大鼠肾组织BMP-7蛋白、mRNA表达,改善早期DN大鼠肾功能病变,延缓DN发展。OBJECTIVE：To explore the effects of puerarin on the pathological change and protein and mRNA expressions of bone morphogenetic protein-7（BMP-7） in the kidney of rats with early diabetic nephropathy（DN）.METHODS：Early DN rat model was induced by streptozotocin（STZ）.The rats were divided into normal control group,model group,enalapril group（1 mg/kg）and puerarin high-dose,medium-dose and low-dose groups（200,100,50 mg/kg）.They were given medicines intragastrically once a day for consecutive 5 weeks.After the last administration,the urinary volume,body weight,24 h urine volume,kidney weight/body weight,FBG,BUN,Scr,insulin（Ins）,24 h urinary protein（Upro）and 24 h urinary microalbumin（UmAlb）were tested;the pathological changes and ultrastructures in renal tissue of DN rats were observed by optical and electron micrograph.Immunohistochemistry and RT-PCR were used to detect the protein and mRNA expressions of BMP-7 in the renal tissue of rats.RESULTS：Compared with model,FBG,KW/BW,BUN,Scr of puerarin high-dose,medium-dose and low-dose groups decreased while BW,Ins level and protein and mRNA expression of BMP-7 increased;the pathological changes and ultrastructureal lesion in renal tissue of DN rats were improved（P〈0.01 or P〈0.05）.24 h urine volume,24 h Upro,24 h UmAlb in Pue high-dose,medium-dose groups decreased（P〈0.01 or P〈0.05）.CONCLUSION：Puerarin can obviously improve renal function and increase the protein and mRNA expression of BMP-7 in kidney tissue,and delay the progression of DN in DN model rats.

关 键 词：葛根素 糖尿病肾病 骨形态发生蛋白-7 病理形态学 超微结构 

分 类 号：R285[医药卫生—中药学] R96[医药卫生—中医学]