道地药材川白芷的质量标准提高研究  

Improvement of Quality Standards of Genuine Regional Drug Angelicae Dahuricae Radix from Sichuan Province

作　　者：唐策[1] 范刚[1] 张艺[1] 杨永东[1] 邝婷婷[1] 万丽[2] 

机构地区：[1]成都中医药大学民族医药学院,成都611137 [2]成都中医药大学药学院,成都611137

出　　处：《中国药房》2013年第3期235-238,共4页China Pharmacy

基　　金：国家科技支撑计划课题资助(No.2007BAI40B05)

摘　　要：目的:为解决因产地加工原因引起的川白芷药材标准不规范、质量差异较大的问题,进行针对性的道地药材川白芷质量标准提高研究。方法:以氧化前胡素、欧前胡素和异欧前胡素为指标,采用薄层色谱(TLC)和高效液相色谱(HPLC)分析方法,建立熏硫和未熏硫川白芷药材的质量评价标准。结果:12批川白芷的TLC中均检出氧化前胡素、欧前胡素和异欧前胡素的特征斑点。氧化前胡素、欧前胡素和异欧前胡素的质量浓度分别在0.00308～0.06154、0.00374～0.07488、0.00290～0.05760mg/ml范围内与各自峰面积积分值呈良好线性关系(r均为0.9999);三者的平均加样回收率分别为100.00%、99.39%、99.81%,RSD分别为1.80%、1.11%、1.41%(n=6)。结论:TLC鉴别方法专属性强;HPLC含量测定方法精密度高、重复性好、简便、快捷,可用于控制川白芷是否熏硫的检测及含量测定,使川白芷质量标准更具有科学性。OBJECTIVE：To solve the problems of non-standard specification and quality differences of Angelicae Dahuricae Radix due to the reason of original processing,and to improve quality standards of Angelicae Dahuricae Radix from Sichuan province.METHODS：Using oxypeucedanin,imperatorin and isoimperatorin as index,the quality standards of Angelicae Dahuricae Radix either sulfured or not were established by TLC and HPLC.RESULTS：The characteristic spots of oxypeucedanin,imperatorin and isoimperatorin of 12 batches of Angelicae Dahuricae Radix were detected by TLC.The linear ranges of oxypeucedanin,imperatorin and isoimperatorin were 0.003 08-0.061 54 mg/ml（r=0.999 9）,0.003 74-0.074 88 mg/ml（r=0.999 9）and 0.002 90-0.057 60 mg/ml（r=0.999 9）separately.The recoveries were 100.00%（RSD=1.80%,n=6）,99.39%（RSD=1.11%,n=6） and 99.81%（RSD=1.41%,n=6）,separately.CONCLUSION：TLC identification is strong specific;and HPLC is high accurate,good reproductive,convenient and rapid,which could be applied to evaluate whether Angelicae Dahuricae Radix sulfured or not and determine effective components,so as to carry out a more scientific method for the quality control of Angelicae Dahuricae Radix.

关 键 词：川白芷 质量标准 薄层色谱法 高效液相色谱法 熏硫 

分 类 号：R284.1[医药卫生—中药学] R917[医药卫生—中医学]