利用RNA干涉研究水稻锌指蛋白基因OsZRL的功能  

Function Analysis of a Rice Zinc Finger Protein Gene OsZRL by RNA Interference

作　　者：玉晓红[1] 晁丽[1] 陈昌冬[1] 刘永胜[1,2] 牛向丽[1,2] 

机构地区：[1]重庆大学生命科学学院,重庆400044 [2]合肥工业大学生物与食品工程学院,安徽合肥230009

出　　处：《中国水稻科学》2013年第1期9-16,共8页Chinese Journal of Rice Science

基　　金：国家自然科学基金资助项目(30971752,30970260);重庆市科技攻关计划资助项目(2010AA1019)

摘　　要：通过RNA干涉对一个水稻锌指蛋白基因OsZRL的功能进行分析。半定量RT-PCR、定量PCR分析结果显示,转基因植株OsZRL基因表达水平显著下调。与野生型相比,OsZRL表达水平下调的转基因株系叶片变大,根系、茎秆更为发达,表明OsZRL的下调对水稻植株的生长有促进作用。OsZRL基因的表达模式和转基因幼苗表型显示OsZRL参与赤霉素、脱落酸信号途径。因而推测锌指蛋白OsZRL是受赤霉素、脱落酸调节的水稻生长发育负调控因子。The function of a rice zinc-finger protein gene（OsZRL） was investigated by RNA interference.The results of semi-quantitative RT-PCR and quantitative PCR analysis indicated that OsZRL expression levels were significantly downregulated in the transgenic plants.The OsZRL-RNAi transgenic lines were characterized by bigger leaves,stronger roots and stems compared to wild type plants,indicating that the suppression of OsZRL had a positive effect on rice growth.The expression pattern and transgenic seedlings phenotypes revealed that OsZRL was involved in gibberellin or abscisic acid signaling pathway.It suggests that zinc-finger protein OsZRL might perform its function of negetive regulator in rice growth through the regulation of gibberellin and abscisic acid.

关 键 词：水稻锌指蛋白 RNA干涉 负调控 赤霉素 脱落酸 

分 类 号：S511[农业科学—作物学]