维生素C对铜孵育的肉鸡原代肝细胞周期及细胞膜电位的影响  

The effect of vitamin C on the cell cycle and membrane potential of the primary hepatocytes in broilers incubated with copper

作　　者：贾雪霞[1] 李静涛[1] 苏荣胜[1] 任常宝[1] 陈龙[1] 郭剑英[1] 王荣梅[2] 唐兆新[1,3] 

机构地区：[1]华南农业大学兽医学院,广州510642 [2]韶关学院英东农业科学与工程学院,广东韶关512005 [3]广东省兽用生物制品技术研究与应用企业重点实验室,广东肇庆510630

出　　处：《黑龙江畜牧兽医》2013年第1期9-11,共3页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家自然科学基金项目(30871900);韶关市科技计划项目(313-140515)

摘　　要：为了研究维生素C对铜孵育的肉鸡原代肝细胞周期及细胞膜电位的影响,试验将全量换液24 h后的原代肝细胞分为正常对照组(Ⅰ组)和10μmol/L Cu2+(Ⅱ组)、30μmol/L Cu2+(Ⅲ组)、50μmol/L Cu2+(Ⅳ组)、50μmol/L维生素C+50μmol/L Cu2+(Ⅴ组)刺激组,于体外刺激后48小时用流式细胞仪测定各组的细胞周期及细胞膜电位。结果表明:30μmol/L和50μmol/L Cu2+能明显引起静止期肝细胞数增多,增殖期肝细胞数减少,细胞膜电位下降(P<0.05),10μmol/L Cu2+组没有出现明显变化。说明维生素C可显著抑制50μmol/L Cu2+引起的变化,对细胞具有保护作用。To investigate the effect of vitamin C on the cell cycle and membrane potential of the primary hepatocytes in broilers incubated with copper, the primary hepatocytes were divided into normal control （group Ⅰ ） and four stimulus groups treated with 10 μmol/LCu^2＋（group Ⅱ ）, 30 μmol/LCu^2＋ （ group Ⅲ ）, 50 μmol/LCu^2＋ （ group Ⅳ ）, and 50 μmol/L Vc 50μmol/LCu^2＋（ group Ⅴ） after changed the medi-um in 24 h. The cell cycles and cell membrane potentials were measured at 48 h by the methods of flow cytometry（FCM）. The result showed that the number of the primary hepatocytes which were treated with 30 μmol/L and 50 μmol/LCu^2＋ was significantly increased in stationary phase, and was significantly decreased in proliferative stage, and the cell membrane potential was decreased（ P 〈 0.05 ）. There was no obvious change in 10 μmol/LCu^2＋. The results indicate that vitamin C can significantly inhibit the changes caused by 50 μmol/LCu^2＋ , it has a pro-tective effect in the cells.

关 键 词：铜 原代肝细胞 细胞周期 细胞膜电位 维生素C 

分 类 号：S831.5[农业科学—畜牧学]