麻杏石甘汤对铜绿假单胞菌生物被膜的影响  

In vitro Effect of MXSGD against Pseudomonas aeruginosa Biofilms

作　　者：王业梅[1] 程惠娟[1] 朱小明[1] 

机构地区：[1]安徽中医学院,安徽合肥230038

出　　处：《时珍国医国药》2012年第12期2961-2963,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金面上项目(No.81173629)

摘　　要：目的研究麻杏石甘汤对铜绿假单胞菌生物被膜的影响。方法微量倍比稀释法测定麻杏石甘汤、阿奇霉素对铜绿假单胞菌的最小抑菌浓度(MIC),棋盘稀释法测定二者协同抗菌作用,MTT法评价二者对铜绿假单胞菌生物被膜SMIC(SMIC50、SMIC80)的影响,镀银染色法观察麻杏石甘汤对铜绿假单胞菌生物被膜形态的影响。结果麻杏石甘汤SMIC50和SMIC80分别为250,1 000 mg.ml-1;500 mg.ml-1的麻杏石甘汤对铜绿假单胞菌生物被膜的形态有显著影响。结论麻杏石甘汤对铜绿假单胞菌生物被膜有较强的抑制作用。Objective To investigate the effect of Maxing Shigan Decoction（MXSGD） against pseudomonas aeruginosa biofilms in vitro.Methods Microdilution method was used to detect minimal inhibitory concentrations（MIC） of MXSGD and Azithromycin.Checkerboard method was used to examine the synersism of MXSGD combined Azithromycin.MTT assay was used to evaluate the effect of MXSGD on biofilms,and microscope was applied to observe biofilms morphology.Results SMIC50 and SMIC80 of MXSGD against pseudomonas aeruginosa biofilms were 250 and 1000 mg·ml-1 respectively.MXSGD showed a significantly inhibitory effect on pseudomonas aeruginosa biofilms morphology at 500mg·ml-1.Conclusion MXSGD can inhibit pseudomonas aeruginosa biofilms in vitro.

关 键 词：生物被膜 铜绿假单胞菌 麻杏石甘汤 

分 类 号：R285.5[医药卫生—中药学]