广东4种钩藤中毛钩藤碱及总碱含量的HPLC、UV测定  

HPLC and UV Determinations of Hirsutine and Total Alkaloids in Four Species Plants of Uncaria Genus in Guangdong Province

作　　者：严愉妙[1] 罗超华[1] 方丽华 雷晓林 刘伟[1] 莫志贤[1] 

机构地区：[1]南方医科大学中医药学院,广东广州510515 [2]东方药林药业有限公司,广东广州510515

出　　处：《时珍国医国药》2012年第12期2974-2977,共4页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.81173581);广东省广州市白云区科技计划项目(No.2011-KZ-61)

摘　　要：目的建立UV、HPLC测定广东4种钩藤植物的总碱以及毛钩藤碱含量的方法,比较不同物种、不同部位间有效成分的含量变化。方法采用HPLC梯度洗脱法测定毛钩藤碱含量,以Hypersil ODS C18(4.6 mm×200 mm,5μm)为色谱柱,甲醇-水(含0.50%三乙胺,冰乙酸调pH 5.35)为流动相,梯度洗脱,进样体积10.00μl,柱温为40.00℃,245 nm波长下检测分析;以UV测定总碱含量,检测波长为245 nm。结果毛钩藤碱在0.02～0.40μg范围内线性关系良好,r=0.999 5,平均回收率分别为96.830%,RSD=1.600%;总碱含量在不同物种、不同部位里存在差异性,其线性范围为2.00～10.00μg/ml,r=0.998 4。结论该法准确,操作简便、快速,重复性较好,精密度较高,可用于对钩藤属植物不同物种、不同部位的总碱以及有效成分毛钩藤碱含量测定。Objective To establish a method for UV and HPLC determinations of total alkaloids and Hirsutine in Uncaria genus and compare their contents among different parts of Uncaria in Guangdong province.Methods Determination of Hirsutine by HPLC,chromatographic column Hypersil ODS（4.6 mm×200 mm,5 μm）,the mobile phase was Methanol-water（containing 0.50% triethylamine,glacial acetic acid adjusted pH5.35） with gradient elution,the injection volume was 10.00 μl and the column temperature was 40℃.Detection wavelength was 245 nm.Determination of the total alkaloid content by UV spectrophotometry,the detection wavelength was 245 nm.Results The calibration curves of Hirsutine was in good linearity over the range of 0.02~0.40 μg（r =0.999 5）.The average recovery rate were 96.830%,with RSD=1.600%.The total alkaloid content in various Uncaria species were different in different parts of Uncaria genus,and the linear range of 2.00 ~ 10.00 μg/ml,r = 0.998 4.Conclusion This method is accurate,simple,rapid,reproducible and high precision which can be used to determine the contents of total alkaloid and Hirsutine for different types and parts of Uncaria genus.

关 键 词：总碱 毛钩藤碱 高效液相色谱法 紫外-可见分光光度法 

分 类 号：R284.2[医药卫生—中药学]