红色毛癣菌中羊毛甾醇含量的高效液相色谱法分析  

Determination of Lanosterol in Mycelium of Trichophyton rubrum by High Performance Liquid Chromatography

作　　者：赵明月[1] 李治建 古力娜.达吾提 斯拉甫.艾白 

机构地区：[1]新疆石河子大学药学院,新疆石河子832000 [2]新疆维吾尔自治区维吾尔医药研究所,新疆乌鲁木齐830049 [3]新疆维吾尔自治区维吾尔医医院,新疆乌鲁木齐830049

出　　处：《时珍国医国药》2012年第12期2980-2981,共2页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.81160557;81060370);新疆维吾尔自治区自然科学基金资助项目(No.2011211B52)

摘　　要：目的用高效液相色谱法建立了红色毛癣菌中羊毛甾醇含量的测定方法。方法样品在24孔板经7 d培养然后进行皂化提取,甲醇定容后进行高效液相定量测定。结果羊毛甾醇的保留时间为12.1 min,在0.1～10.0 mg/L浓度范围内,峰面积与浓度呈良好的线性关系,其相关系数为0.998 1。羊毛甾醇的平均添加回收率为93.7%,相对标准偏差为1.9%,其最低检出浓度为0.05 mg/L。结论该方法简便、准确、重现性较好,可用于红色毛癣菌中羊毛甾醇的含量测定。Objective To establish a determination method of lanosterol in mycelium of Trichophyton rubrum by reversed phase high performance liquid chromatography.Methods The sample was analyzed after saponifing,extracting and methanol voluming.Results The retention time of lanosterol was 12.1 min.There was excellent linearity between peak area and concentration of analyte in the concentration range of 0.1~10.0 mg/L for lanosterol.The correlation coefficient was 0.998 1.The average recovery was 93.7%.The relative standard deviation was 1.9%.The detection limit of concentration was 0.05 mg/L.Conclusion This method is accurate,simple and precise.

关 键 词：高效液相色谱法 红色毛癣菌 羊毛甾醇 

分 类 号：R284.1[医药卫生—中药学]