栀子药材中京尼平苷含量的薄层-紫外分光光度法测定  

Determination of Geniposide in Gardenia jasminoides Ellis by TLC-UC Spectrophotometry

作　　者：赵成城[1] 刘昊[2] 杨军宣[1] 张海燕[2,3] 杨明[1,2] 

机构地区：[1]成都中医药大学,四川成都611137 [2]江西中医学院现代中药制剂教育部重点实验室,江西南昌330004 [3]西南交通大学材料先进技术教育部重点实验室,四川成都610003

出　　处：《时珍国医国药》2012年第12期2982-2983,共2页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.2009ZX09103-393)

摘　　要：目的建立薄层-紫外分光光度(TLC-UV)法测定栀子药材中京尼平苷的含量。方法在25℃测定京尼平苷在最大吸收波长238 nm处的吸光度。结果此法线性范围6.4～38.4μg/ml内,r=0.999 1,平均回收率为97.68%,RSD=2.72%(n=6)。结论用此方法可测定栀子中京尼平苷的含量。方法简便,灵敏和准确,结果可靠。Objective To determine the content of geniposide in Gardenia jasminoides Ellis by TLC-UV Spectrophotometry.Methods Geniposide was determined at 238 nm with UV spectrophotometry method.Results The average recovery was 97.68 % and RSD=2.72 %,and there was a good linear relationship between mass of geniposide and absorbance within the range of 6.4~38.4 μg/ml（r=0.999 1）.Conclusion The method is simple,sensitive and accurate.

关 键 词：栀子 京尼平苷 薄层-紫外分光光度法 

分 类 号：R284.1[医药卫生—中药学]