铁包金药材中槲皮素的薄层鉴别与含量测定研究  

TLC Identification and Determination of the Quercetin in Berchemia lineata

作　　者：郭力城[1,2] 滕红丽[1,2] 梅之南[3] 

机构地区：[1]广西壮族自治区民族医药研究院 [2]广西壮医医院,广西南宁530001 [3]中南民族大学药学院,湖北武汉430074

出　　处：《时珍国医国药》2012年第12期2983-2985,共3页Lishizhen Medicine and Materia Medica Research

基　　金：广西壮族自治区千亿元产业重大科技攻关(No.桂科攻11107009-2-1);广西壮族自治区自然科学基金(No.0991006);国家中医药管理局中医药专项资金(No.2010MZWXGX09);广西壮族自治区中医药管理局科技专项(No.GZYZ1114);广西民族医药创新研究与产业化技术平台的建设(No.10124008-15)资助项目

摘　　要：目的对铁包金药材中槲皮素进行薄层鉴别并建立铁包金药材中槲皮素的含量测定方法。方法采用薄层色谱法对铁包金药材中槲皮素进行鉴别并采用高效液相色谱法进行含量测定,色谱柱为迪马ODS C18(4.6 mm×250 mm,5μm);流动相为乙腈-甲醇(5∶1)A:0.1%磷酸溶液B(梯度:0～30 min,20%～60%A);流速0.85 ml/min。检测波长360 nm,柱温30℃。结果薄层色谱可鉴别槲皮素。槲皮素在0.69～4.83μg范围内线性关系良好,r=0.999 8(n=7),平均回收率为99.8%(RSD:1.27%,n=6)。结论该此法简便、准确,可用于铁包金药材的质量控制。Objective To identify the thin layer of quercetin in Berchemia lineata and set up its content determination method.Methods To identify the quercetin in Berchemia lineate by adopting thin-layer chromatography;and to determine the content by high performance liquid chromatography.The chromatographic column was Diamonsil ODS C18（4.6 mm×250 mm,5μm） and the mobile phase was acetonitrile-methanol（5：1）A∶0.1% phosphoric acid solution B（Gradient∶0~30 min,20~60%A）.The flow velocity,detection wavelength and the column temperature were 0.85 ml/min,360nm and 30℃ respectively.Results Thin-layer chromatography could identify the quercetin.The quercetin showed a good linearity within the range of 0.69~4.83μg,r=0.999 8（n=7） and the average recovery rate was 99.8%（RSD：1.27%,n=6）.Conclusion This method is simple,convenient and accurate and it can be used to control the quality of Berchemia lineate medicinal materials.

关 键 词：铁包金 槲皮素 薄层色谱法 高效液相色谱法 

分 类 号：R284.2[医药卫生—中药学]