栀子药材中总绿原酸含量的紫外测定方法学研究  

Methodology for Determination of Total Chlorogenic Acid in Fructus Gardeniae

作　　者：刘昊[1] 芦乾[1] 宋伟[1] 魏绍峰[1] 王粟莙[1] 

机构地区：[1]江西中医学院现代中药制剂教育部重点实验室,江西南昌330004

出　　处：《时珍国医国药》2012年第12期2997-2998,共2页Lishizhen Medicine and Materia Medica Research

基　　金：国家"十一五"国家重大新药创制科技重大专项(No.2009ZX09103-393)

摘　　要：目的建立栀子中总绿原酸含量的紫外测定方法。方法测定栀子中总绿原酸在波长300 nm处的吸光度。结果此法在6～30μg/ml浓度范围内,吸光度与浓度线性关系良好,Y=30.837X-0.014,r=0.999 6,平均回收率为101.37%,RSD=1.96%。结论紫外测定栀子总绿原酸含量,方法简单易行,快速准确,能用于栀子药材的质量控制。Objective The article aim to establish UV method for assaying total chlorogenic acids in GF.Methods Determining the absorbance of total chlorogenic acids in GF at 300 nm.Results The linear relationship between absorbance and concentration was good within the range of 6~30 μg/ml with R=0.9996,Y=30.837X-0.014.The average recovery was 101.37%,RSD within 1.96%.Conclusion This method is simple,quick and accurate,and can be used for GF quality control.

关 键 词：栀子 总绿原酸 紫外-可见分光光度法 

分 类 号：R284.1[医药卫生—中药学]