微生物转化生产S-腺苷甲硫氨酸  

S-adenosyl-L-methionine Production by Microorganism

作　　者：胡晓清 郭雯 韩国强 

机构地区：[1]江南大学食品科学与技术国家重点实验室江南大学生物工程学院,无锡214122

出　　处：《生物技术通报》2012年第12期33-39,共7页Biotechnology Bulletin

基　　金：国家重点基础研究发展计划项目(2012CB725202)

摘　　要：S-腺苷甲硫氨酸(S-adenosyl-L-methionine,SAM)是具有广阔市场前景的活性氨基酸,微生物转化法是近年来报道较多的SAM生产方法。综合近年来SAM生产菌株的基因改造和发酵优化方面的进展,从提高SAM合成酶表达和酶活、优化甲醇和甘油的流加方式、改善ATP的生成和L-甲硫氨酸的补料、阻断下游代谢路径等方面,综述了促进SAM合成及其积累的多重策略及机制。最后结合笔者多年研究实践,讨论了微生物转化生产SAM的未来研究方向。S-adenosyl-L-methionine （ SAM ） is a type of active amino acid having broad market prospects, and there have been many reports on SAM production catalyzed by microorganisms. This paper integrated advances in genetic modification and fermentation optimization of SAM-producing strain. From the points of elevating expression and activity of SAM synthetase, optimizing feeding mode of glycerol and methanol, improving the generation of ATP and supplement policy of L-methionine, and blocking downstream metabolic pathway, the multiple strategies of enhancing SAM synthesis and accumulation and their mechanisms had been reviewed. Furthermore, on the basis of the authors＇ research practice, some new prospects on SAM biosynthesis by microbes were discussed.

关 键 词：S-腺苷甲硫氨酸 微生物转化 S-腺苷甲硫氨酸合成酶 基因改造 发酵优化 

分 类 号：TQ463[化学工程—制药化工]