稻属单拷贝核基因TPI序列分析及其在疣粒野生稻鉴定中的应用  

Analysis of Single-copy Nuclear TPI Gene Sequences of Oryza L.and Its Application in Identification of Oryza granulata

作　　者：宋云[1] 陈岩[1] 徐晗[1] 李明福[1] 刘仕琴[2] 

机构地区：[1]中国检验检疫科学研究院植物检疫研究所,北京100029 [2]中国科学院昆明植物研究所,昆明650204

出　　处：《生物技术通报》2012年第12期76-81,共6页Biotechnology Bulletin

基　　金：国家质检总局科技计划项目(2012IK295);国家科技支撑计划项目(2012BAK11B01);中国检验检疫科学研究院基本科研业务费专项(2012JK004)

摘　　要：通过对稻属(Oryza L.)及其近缘属等18个禾本科植物单拷贝核基因TPI序列进行扩增和测序,分析其系统进化关系及序列差异,并设计鉴定疣粒野生稻(O.granulata)特异的分子标记。序列分析表明,栽培稻间TPI基因序列碱基变异少,野生稻相对变异丰富,其中疣粒野生稻变异尤为明显,根据疣粒野生稻的TPI基因序列特异位点设计引物,利用这一分子标记对疣粒野生稻进行准确鉴定。In this paper, single-copy nuclear TPI genes of Oryza L. and its related genera were amplified and sequenced, we analyzed their phylogenetic relationships and sequence differences, designed specific primers for Oryza granulata. Sequences analysis indicate that there were few variations between cuhivar rice, but many variations between wild rice species, especially sharp variations were found for Oryza granulata. On the basis of these variations, we designed Oryza granulata specified primers for species identification.

关 键 词：TPI基因 疣粒野生稻 分子标记 物种鉴定 

分 类 号：S511.9[农业科学—作物学]