建兰Actin基因cDNA全长的克隆及其表达分析  

Cloning and Expression Analysis of CeActin Homologous Gene from Cymbidium ensifolium

作　　者：吴菁华[1] 吴少华[1] 杨超[1] 

机构地区：[1]福建农林大学园艺学院,福州350002

出　　处：《生物技术通报》2012年第12期88-92,共5页Biotechnology Bulletin

基　　金：福建省自然科学基金项目(2009J01066)

摘　　要：根据单子叶植物的肌动蛋白基因(Actin)的保守区序列设计引物,采用RT-PCR和RACE技术从建兰(Cymbidiumensifolium)中分离出Actin基因cDNA全长。序列分析结果表明,建兰Actin基因长度为1 434 bp,编码区长度为1 134 bp,编码377个氨基酸,将其命名为CeActin,GenBank登录号为JN613147。CeActin推导的氨基酸序列与其他植物的同源性都较高,具有高度的保守性。采用半定量RT-PCR技术分析CeActin在建兰各组织及花不同发育时期的表达情况,结果表明,表达量没有明显差异,表明CeActin基因可作为内参基因。The full-length cDNA sequence of Actin gene from the bud of Cymbidium ensifolium was cloned （ GenBank accession number ： JN613147 ） by using RT-PCR and RACE with degenerate primer which were synthesized based on the high homologous region among the sequences of several monocotyledon Actin genes. The full length cDNA of CeActin was 1 434 bp with an ORF of 1 134 bp encoding a protein with 377 amino acids. Homology search showed that this gene shared high identity with other Actins. Semi-quantitative RT-PCR analysis showed that the expression of CeActin had no notable difference in different tissues and different floral development stages. The results laid basis for identification of gene transcription level in Cymbidium ensifolium using CeActin as internal control.

关 键 词：建兰 肌动蛋白基因 克隆 表达分析 

分 类 号：S682.31[农业科学—观赏园艺]