河南省部分地区溴氰菊酯抗性中华按蚊代谢酶活性的调查研究  

Investigation of activity of metabolic enzymes in deltamethrin-resistant Anopheles sinensis from some regions of Henan province,China

作　　者：祁欣[1,2] 张红卫[3] 徐铁龙[1] 汤林华[1] 周水森[1] 郑彬[1] 

机构地区：[1]中国疾病预防控制中心寄生虫病预防控制所,卫生部寄生虫与病原生物学重点实验室,世界卫生组织疟疾,血吸虫和丝虫病合作中心,上海200025 [2]中国检验认证集团河南有限公司,河南郑州450008 [3]河南省疾病预防控制中心

出　　处：《中国媒介生物学及控制杂志》2012年第6期536-538,共3页Chinese Journal of Vector Biology and Control

摘　　要：目的了解河南省部分地区溴氰菊酯抗性中华按蚊的代谢酶活性,探讨中华按蚊对溴氰菊酯的代谢抗性机制。方法 2010年8月在河南省桐柏、淮滨县和永城市采集中华按蚊样本,子1代成蚊用接触筒内成蚊药膜滤纸接触法,按照WHO标准,区分溴氰菊酯抗性与敏感样本,并用微量滴定板法测定抗性样本的非特异性酯酶、谷胱甘肽S转移酶(GST)和P450单加氧酶活性。以实验室敏感品系蚊为对照。结果淮滨、永城和桐柏三地中华按蚊抗性样本的A、B型非特异性酯酶活性均高于对照组(t=2.41,3.30,10.31,7.67,7.90,11.17,均P<0.05);永城和淮滨地区中华按蚊抗性样本GST活性高于对照组的敏感品系(t=3.687,2.484,均P<0.05);淮滨、永城及桐柏三地的中华按蚊抗性样本和敏感品系的P450单加氧酶活性的差异均无统计学意义(t=-1.489,0.397,-0.413,均P>0.05)。结论永城、淮滨两个地区中华按蚊的溴氰菊酯抗性与酯酶和GST活性增高有关;桐柏地区中华按蚊的溴氰菊酯抗性与酯酶活性增高有关。Objective To investigate the activity of metabolic enzymes in Anopheles sinensis from some regions in Henan province,China and the mechanism of metabolic resistance to deltamethrin in An.sinensis.Methods An.sinensis samples were collected from Tongbai county,Huaibin county,and Yongcheng city（county-level） in Henan province in August 2010.The adult mosquitoes of the next generation were divided into deltamethrin-resistant group and deltamethrin-sensitive group by filter contact method according to WHO standard.The activity of metabolic enzymes in deltamethrin-resistant mosquitoes,including nonspecific esterase,glutathione S-transferase（GST）,and P450 monooxygenase,was measured by microtiter plate method.The laboratory sensitive strain of An.sinensis was used as the control.Results The activity of types A and B nonspecific esterases was significantly higher in the deltamethrin-resistant samples from Tongbai,Huaibin,and Yongcheng than in the control group（t=2.41,t=3.30,t=10.31,t=7.67,t=7.90,t=11.17,P〈0.05 for all comparisons）.The activity of GST in the deltamethrin-resistant samples from Yongcheng and Huaibin was higher than that in the control group（t=3.687,t=2.484,P〈0.05 for both comparisons）.There were no significant differences in the P450 monooxygenase activity between the deltamethrin-resistant samples from Huaibin,Yongcheng,and Tongbai and the control group（t=-1.489,t=0.397,t=-0.413,P〈0.05 for all comparisons）.Conclusion The deltamethrin resistance in An.sinensis from Yongcheng and Huaibin are related to the increased activity of esterase and GST,but the deltamethrin resistance in An.sinensis from Tongbai is only related to the increased activity of esterase.

关 键 词：溴氰菊酯 中华按蚊 非特异性酯酶 谷胱甘肽S转移酶 P450单加氧酶 

分 类 号：R384.1[医药卫生—医学寄生虫学] S482.3[医药卫生—基础医学]