肝细胞生长因子基因治疗促进海水浸泡伤创伤愈合的实验研究  

HGF gene therapy to promote wound healing of injury with seawater immersion:an experimental study

作　　者：张达矜[1] 乔媛媛[1] 王大鹏[1] 解鹏[1] 何立东[1] 毕建进[2] 吴祖泽[2] 崔春萍[2] 赵晓航[1] 

机构地区：[1]中国人民解放军海军总医院基础医学研究中心,北京100048 [2]军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《军事医学》2012年第12期903-906,共4页Military Medical Sciences

基　　金：国家自然科学基金资助项目(31071256;30800558;81070330;30930041)

摘　　要：目的观察肝细胞生长因子(HGF)基因治疗对海水浸泡伤创伤愈合的影响。方法将20只20周龄健康雄性Wistar大鼠随机均分为单纯创伤组、创伤合并海水浸泡(创伤+海浸)组和3个给药组(0μg、20μg、40μg)。建立双侧切割伤合并海浸大鼠模型,采用INJEX无针注射器紧贴创口边缘皮肤,每创口4点注射HGF重组质粒(pUDKH)。每天观察伤口愈合情况,并测量和计算创面面积,分别于第1、6、12和16天伤口取材,HE染色后进行病理学观察。结果从致伤后第2天开始各时间点,20μg和40μg pUDKH给药组大鼠创口面积均明显小于创伤+海浸组和0μg给药组。组织病理学观察结果显示,与创伤+海浸组和0μg给药组相比,20μg和40μgpUDKH给药组于致伤后第1天的创伤部位炎性反应较轻,于致伤后第6天的肉芽组织和新生血管生长增加,再上皮化进程较早,创面愈合提前2～3 d。结论 HGF基因治疗能够促进海水浸泡伤创伤愈合,其作用可能与HGF抑制伤口局部炎症反应,促进新生血管生长和再上皮化过程等有关。Objective To study the effect of gene therapy of human hepatocyte growth factor （HGF） on wound healing of injury with seawater immersion. Methods Twenty healthy Wistar rats of 20 weeks old were divided randomly into five groups, including injury group, injury with seawater immersion group and 0 μg, 20μg and 40 μg treated groups. A rat model of bilateral incised wounds accompanied by seawater immersion was used. The recombinant plasmids carrying human hepatocyte growth factor gene pUDKH were injected at 4 points around wounds by an INJEX needleless injection system. The healing process was observed each day and the size of the wound surface was measured. Wound tissues were obtained at d 1 ,d 6, d 12 and d 16, and studied by pathological analysis after HE staining. Results Compared with injury with seawater immersion group and 0 μg treated group, the size of wound surface in rats of both 20 μg and 40 μg treated groups was apparently smaller at each time poins since d 2. When the test animals were treated with pUDKH, the relief of the inflammatory reaction was observed at d 1, and improvement of neovascularization, granulation tissue and the process of re-epithelization were observed at d 6. The total time of wound healing was shortened by 2 or 3 days in the rats of the 20μg and 40 μg pUDKH treated groups compared with the injury with seawater immersion and the 0 Ixg treated groups. Conclu- sion HGF gene therapy may promote wound healing of injury accompanied by seawater immersion. The function of HGF is to inhibit inflammation and to promote the formation of neovascularization and re-epithelization.

关 键 词：肝细胞生长因子 海水 基因治疗 创伤和损伤 

分 类 号：R641[医药卫生—外科学]