体外成熟卵母细胞体外受精方式的探讨  

A Study on the Approach of In Vitro Fertilization for Mature Oocytes from In Vitro Maturation

作　　者：周桦[1] 周从容[1] 

机构地区：[1]贵阳医学院附属医院生殖医学中心,贵阳550004

出　　处：《生殖与避孕》2013年第1期55-57,共3页Reproduction and Contraception

摘　　要：目的:探讨经体外成熟卵母细胞是否可采用常规IVF方式授精。方法:自愿捐献的常规废弃的未成熟卵母细胞经体外成熟后的卵母细胞206个,随机分为3组:A组(n=69),授精时尽可能保存卵丘细胞并行常规IVF授精;B组(n=68),授精前将卵丘细胞完全拆除后行常规IVF授精;C组(n=69),将卵丘细胞完全拆除后行ICSI授精。常规IVF授精的精/卵个数比约为50 000∶1。比较3组卵子的受精率、卵裂率和优质胚胎率。结果:A组与C组在受精率(82.61%vs 85.51%)、卵裂率(94.74%vs 94.92%)、优质胚胎率(42.59%vs 46.43%)3个方面均无统计学差异(P>0.05),A组、C组的受精率、卵裂率均显著高于B组(14.71%,50.00%)(P<0.01),B组没有优质胚胎出现。结论:体外成熟后的卵母细胞在卵丘细胞较完整时可进行常规IVF方式授精。Objective： To investigate the approach of /n vitro fertilization for mature oocytes from in vitro maturation. Methods： A total of 206 mature oocytes from in vitro maturation （IVM） of scrap immature oocytes were selected and divided into 3 groups. Group A （n=69）： all of the oocytes were chosen for routine in vitro fertilization （IVF） and their oocyte cumulus complex （OCC） were remained for the best; group B （n=68）： all of the oocytes were chosen for routine IVF but their OCC were cut off; group C （n=69）： all of the oocytes were chosen for intracytoplasmic sperm injection.The ratio of sperm/oocyte was about 50 000 2 1 in routine IVF. Rates of fertilization, cleavage, and top-quality embryo were compared in the 3 groups. Results： There was no statistical difference in rates of fertilization （82.61% vs 85.51%）, cleavage （94.74% vs 94.92%）, and top-quality embryo （42.59% vs 46.43%） between group A and group C （P〉0.05）, but the rates of fertilization （14.71%） and cleavage （50.00%） in group B were significantly lower when compared with group A and group C （P〈0.01）, there was no top-quality embryo in group B. Conclusion： The mature oocytes from IVM can be fertilized in routine IVF when they had complete OCC.

关 键 词：未成熟卵母细胞 体外成熟培养(IVM) 体外授精(IVF) 卵丘细胞 

分 类 号：R714.8[医药卫生—妇产科学]