拟穴青蟹(Scylla paramamosain)PP2A调节亚基B的基因克隆与表达分析  

CLONING AND EXPRESSION ANALYSIS OF THE REGULATORY SUBUNIT B GENE OF PP2A IN THE MUD CRAB SCYLLA PARAMAMOSAIN

作　　者：刘学良[1] 金朱兴[1] 黄辉洋[1] 叶海辉[1] 李少菁[1] 

机构地区：[1]厦门大学海洋与地球学院,厦门361005

出　　处：《海洋与湖沼》2012年第5期932-937,共6页Oceanologia Et Limnologia Sinica

基　　金：国家自然科学基金资助项目;40406030号;41076081号

摘　　要：采用RT-PCR、RACE等方法,获得了拟穴青蟹PP2A调节亚基B(PP2A-B)的cDNA全序列,全长2040bp,开放阅读框(ORF)为1332bp,可编码443个氨基酸残基。同源分析显示,该基因编码的蛋白与其它一些物种具有很高相似性,推测PP2A-B基因具有很高的保守性。经荧光定量PCR检测,PP2A-B基因在拟穴青蟹多个组织中有表达,且在脑、卵巢、鳃中表达量较高。在拟穴青蟹卵巢发育过程中,PP2A-B基因在卵巢未发育期(I期)表达量最高,此后各期逐渐下降,推测PP2A-B在卵巢中可能以PP2A全酶的形式存在,抑制卵巢发育。In this paper, the regulatory subunit B gene of PP2A （PP2A-B） was isolated from the mud crab, Scylla pa- ramamosain using RT-PCR and RACE methods. The obtained full-length cDNA of PP2A-B was 2040bp with an open reading frame of 1332bp encoding a putative peptide of 443 amino acid. By alignment, the amino acid sequence of S. pa- ramamosain PP2A-B showed high homology with those of some other animals. It suggested PP2A-B was highly conserva- tive. Real-time PCR showed that the PP2A-B gene was expressed in various tissues, and highly expressed in brain, ovary and gill. The PP2A-B mRNA expression during ovarian development indicated that the expression of PP2A-B was obvi- ously high at undeveloped stage and decreased gradually from stage I （undeveloped stage） to stage V （ripe stage）. We hy- pothesized that PP2A-B may exist in the form of PP2A holoenzyme in ovary and play a suppressive role on the ovarian development of the mud crab.

关 键 词：PP2A-B 拟穴青蟹 基因克隆 荧光定量PCR 卵巢发育 

分 类 号：S917.4[农业科学—水产科学]