不同水温下儿茶素EGCG在黄颡鱼(Pelteobagrus vachelli)体内代谢动力学和肝消除研究  

METABOLIC KINETICS AND LIVER ELIMINATION OF EPIGALLOCATECHIN GALLATE(EGCG) IN DARKBARBEL CATFISH PELTEOBAGRUS VACHELLI AT DIFFERENT TEMPERATURE

作　　者：葛建[1,2] 刘洋[1] 李明揆[1] 赵进[1] 韩宝瑜 

机构地区：[1]中国计量学院生命科学学院,杭州310018 [2]浙江省生物计量及检验检疫技术重点实验室,杭州310018

出　　处：《海洋与湖沼》2012年第5期938-942,共5页Oceanologia Et Limnologia Sinica

基　　金：国家自然科学基金资助项目;31100499号;31071744号;浙江省自然科学基金资助项目;Y3080255号;浙江省分析测试计划项目;2011C37044号

摘　　要：采用反相高效液相色谱-紫外检测法测定了黄颡鱼血浆、肝脏中儿茶素EGCG含量,并研究了不同水温(10℃和20℃)下EGCG在黄颡鱼体内代谢动力学和肝组织消除特征。结果表明,高温下黄颡鱼腹腔注射EGCG后体内吸收较快,T1/2ka为0.21h,T1/2α为2.07h,T1/2β为22.89h,AUC为5195.41μg·h/ml,Tp为0.95h,Cmax为257.75μg/ml;低温下分别为5.67h、9.53h、207.49h、17283.03μg·h/ml、12.56h和117.16μg/ml。同时在0.5、1、2、4、8、12、24、48、96h各时间点检测了肝脏组织中EGCG浓度,并计算得出高温下EGCG在肝组织中的T1/2β为24.15h,低温下肝组织中T1/2β为64.17h。对不同水温下代谢动力学和肝组织消除规律的研究表明,EGCG在黄颡鱼体内吸收、分布和消除速度与水温关系密切,高温下均较低温下迅速。The epigallocatechin gallate （EGCG） concentrations in plasma and liver tissues were determined by the re- versed-phase high performance liquid chromatography （RP-HPLC） after a single intraperitoneal injection at 150mg/kg for Darkbarbel catfish, Pelteobagrus vachelli. The results showed that EGCG was absorbed and eliminated very quickly in high temperature （20℃）. However, EGCG was absorbed and eliminated very slowly in low temperature （10℃）. The T1/2Ka, T1/2a and T1/2β were 0.21h, 2.07h and 22.89h at 20℃, respectively. And the corresponding values were 5.67h, 9.53h and 207.49h at 10℃, respectively. The AUC, Tp and Cmax were 5195.41gg.h/ml, 0.95h and 257.75gg/ml at 20℃. Then all the values were 17283.031ag.h/ml, 12.56h and 117.16gg/ml at 10℃, respectively. The elimination half-life of EGCG in liver was 24.15h at 20℃, while the value was 64.17h at 10℃. Therefore, these results indicate that EGCG absorption, distribu- tion and excretion at 20℃ were more quickly than at 10℃. And the differences of metabolic kinetics and liver elimination of EGCG were significant at 20℃ and at 10℃.

关 键 词：黄颡鱼 儿茶素EGCG 反相高效液相色谱 代谢动力学 肝脏消除 

分 类 号：X503.225[环境科学与工程—环境工程]