泥蚶(Tegillarca granosa)遗传连锁图谱的构建  被引量:2

CONSTRUCTION OF GENETIC LINKAGE MAP OF TEGILLARCA GRANOSA LINNAEUS

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作  者:刘博[1,2] 滕爽爽[1,2] 柴雪良[1,2] 邵艳卿[1,2] 肖国强[1,2] 张炯明[1,2] 方军[1,2] 

机构地区:[1]浙江省海洋水产养殖研究所,温州325000 [2]浙江省近岸水域生物资源开发与保护重点实验室,温州325000

出  处:《海洋与湖沼》2012年第5期983-991,共9页Oceanologia Et Limnologia Sinica

基  金:国家高技术研究发展计划(863计划);2012AA10A410-1号;浙江省自然科学基金项目;Y3110003;浙江省科研院所专项;2009F20010号;温州市科技兴海项目;S20080019号

摘  要:采用AFLP标记技术结合"拟测交"策略,对泥蚶父、母本和109个F1全同胞个体进行了遗传连锁的分析研究,构建了泥蚶的遗传连锁图谱。结果表明,在638个分离标记中,符合1:1孟德尔分离类型位点的母本和父本分离标记分别为235和259个;3:1孟德尔分离类型位点59个。雌性图谱包括21个连锁群,标记间平均间隔为8.12cM,图谱共覆盖1737.8cM,覆盖率为83.1%;雄性图谱包括20个连锁群,标记间平均间隔为5.81cM,图谱共覆盖1388.2cM,覆盖率为84.7%。Based on segregation of amplified fragment length polymorphism (AFLP) markers, the first genetic linkage map of Tegillarca granosa Linnaeus was constructed using the “two-way pseudo-testcross” strategy. A segregation popula- tion (F1) including 109 progenies (full-sib family) from the cross between two wild populations of T. granosa from Jiangsu and Yueqing was obtained. The parents and the F1 progeny were used as the segregation population. A set of 66-pair primer combination were used to analyze the parents and the 109 progenies of the mapping family. A total of 638 segregation sites were identified. Among them, 494 (235 for female and 259 for male) fitted in the expected 1 : 1 Mendelian segregation ratio and 59 fitted in a 3 : 1 Mendelian ration. The female linkage maps included 21 linkage groups, spanning 1737.8cM with the average marker density of 8.12cM, and the observed coverage was 83.1%. The male linkage maps included 20 linkage groups, spanning 1388.2cM with the average marker density of 5.81cM, and the observed coverage was 84.7%.

关 键 词:泥蚶 AFLP 遗传连锁图谱 拟测交策略 

分 类 号:Q75[生物学—分子生物学]

 

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