小鼠硫酸黏杆菌素中毒神经电生理时效变化  

Time-dependent changes in electrophysiology of mice sciatic-tibial nerve caused by Colistin Sulfate

作　　者：代重山[1] 李继昌[1] 马晶[2] 高瑞霞[3] 

机构地区：[1]东北农业大学动物医学学院,黑龙江哈尔滨150030 [2]东北农业大学医院,黑龙江哈尔滨150030 [3]东北农业大学生命学院,黑龙江哈尔滨150030

出　　处：《中国兽医杂志》2012年第12期11-14,共4页Chinese Journal of Veterinary Medicine

基　　金：黑龙江省留学归国科学基金项目(LC201018);教育部留学回国人员科研启动基金资助项目(41);黑龙江省教育厅科学技术项目(12521043)

摘　　要：昆明系雌性小鼠尾静脉注射硫酸黏杆菌素7.5mg/kg体重.12h,对照组注射生理盐水,连续7d,分别于给药后1,3,7,15d观察小鼠的步态、体重、坐骨-胫神经的电生理指标变化,探究硫酸黏杆菌素中毒小鼠坐骨-胫神经动作电位时间-效应关系及敏感性指标。结果显示,黏杆菌素给药后1,3,7,15d与对照相比,坐骨-胫神经动作电位各指标随各时间点发生变化,阈值(TP)增加,分别为59.8%,60.5%,192.5%(P<0.01),70.1%;最大刺激强度(MI)增加,分别为3.63%,12.7%,100%(P<0.05),29.1%;时程(CAPD)增加,分别为15.1%,11.5%,52.8%(P<0.05),20.6%;潜伏期(CAPL)增加,分别为9.0%,9.0%,14.6%(P<0.05),11.2%;波幅(CAPA)降低,分别为0.92%、16.2%、47.6%(P<0.01)、38.8%(P<0.05);传导速率(NCV)减低,分别为7.6%,7.5%,12.7%(P<0.05),9.3%;给药后在第1天出现重复性动作电位。结果证实,硫酸黏杆菌素中毒后,小鼠坐骨-胫神经电生理改变存在时效性变化,CAPA较为敏感。To investigate the time-dependent electrophysiological changes and electrophysiological sensitive pa- rameters of the sciatictibial nerves of mice treated with colistin sulfate. Colistin sulfate （7. 5 mg/kg/12 h） was administered intravenously to female mice for 7 days and the control groups received normal saline. The gait was observed and electrophysiological parameters of mice sciatic-tibial nerves were determined o d 0 1, 3, 7 and 15. The results showed that, compared with the control, on d 1, 3 ,7 and 15, the threshold intensities （TP） were in- creased by 59.8%, 60.5%, 192.5% （P〈0.01） and 70. 1%, respectively; the maximal intensities （MI） were in- creased by 3.63%, 12.7%, 100% （P〈0.05） and 29.1 %, respectively; the compound action potential durations （CAPD） were increased by 15.1%, 11.5%, 52.8% （P〈0.05） and 20. 6%, respectively; the latencies of com- pound action potential（CAPL） were prolonged by 9.0%, 9.0%, 14. 6% （P〈0. 05） and 11.2%, respectively; the compound action potential amplitudes （CAPA） decreased 0.92%, 16.2%, 47.6%（P%0.01） and 38. 8% （P 〈0.05）, respectively; and the conduction velocities （NCV） decreased by 7.6%, 7.5%, 12.7% （P〈0.05） and 9.3 %, respectively. Repetitive CAP was found only on day 1. These results confirmed that the electrophysiological changes of the sciatictibial nerves in mice treated with colistin sulfate were in a time-dependent manner. CAPA were more sensitive than others.

关 键 词：硫酸黏杆菌素 坐骨-胫神经 行为学测试 电生理学 

分 类 号：S816.3[农业科学—饲料科学]