川芎嗪通过Akt信号通路影响前列腺癌PC3细胞的增殖和凋亡  

Tetramethylpyrazine hydrochloride inhibits proliferation and apoptosis in human prostate cancer PC3 cells through Akt signaling pathway

作　　者：韩娇艳[1] 朱方强[2] 徐祥[3] 黄宏[3] 黄文秋[1] 崔文慧[1] 代卉[1] 蒋建新[4] 王莎莉[1] 

机构地区：[1]重庆医科大学神经科学研究中心,重庆400016 [2]第三军医大学西南医院肾内科,重庆400038 [3]第三军医大学大坪医院野战外科研究所第一研究室,创伤,烧伤与复合伤国家重点实验室,重庆400042 [4]第三军医大学大坪医院野战外科研究所第四研究室,创伤,烧伤与复合伤国家重点实验室,重庆400042

出　　处：《第三军医大学学报》2013年第2期105-108,共4页Journal of Third Military Medical University

基　　金：国家重点基础研究发展计划(973计划;2012CB518104);国家重点实验室基金(SKLZZ200907);重庆市科委回国人员启动基金(CSTC2010BB5036)~~

摘　　要：目的研究盐酸川芎嗪对前列腺癌PC3细胞增殖和凋亡的影响及作用机制。方法 0、0.2、0.5、1.0、1.5、2.0、2.5、3.0、3.5 mg/mL盐酸川芎嗪处理雄激素非依赖性前列腺癌PC3细胞,MTT法检测各组细胞的增殖能力,荧光显微镜观察细胞核形态学改变,Western blot检测Akt以及下游相关蛋白mTOR、p70S6蛋白及蛋白磷酸化的表达,以及凋亡相关蛋白Bcl-2和Bax的表达。结果盐酸川芎嗪能有效抑制前列腺癌PC3细胞增殖,且显示浓度时间依赖性(P<0.05)。1.5、2.5 mg/mL的盐酸川芎嗪能够降低Akt和p-Akt的表达,进而下调mTOR、p-mTOR、p70S6及p-p70S6的表达(P<0.05);同时下调Bcl-2、上调Bax蛋白的表达(P<0.05)。结论 Akt及其下游信号通路介导了盐酸川芎嗪抑制前列腺癌PC3细胞增殖及促凋亡作用。Objective To investigate the inhibitory effect of 2,3,5,6- tetramethylpyrazine hydrochlo- ride （TMP） on the proliferation and apoptosis in human prostate cancer PC3 cells and to explore its underlying mechanism. Methods Different concentrations of TMP （0, 0.2, 0.5, 1.0, 1.5, 2.0, 2.5, 3.0 and 3.5 mg/mL） was added to treat PC3 cells. MTT assay was performed to test the inhibitory effect on cell proli- feration, and fluorescence microscopy was applied to test the nuclear alteration. Western blot assay was used to detect the expression of Akt and the related downstream targets. Results TMP inhibited the proliferation in PC3 cells in a dose- and time-dependent manner（P 〈0. 05）. TMP of 1.5 and 2.5 mg/mL decreased the protein levels of Akt, p-Akt, as well as the protein level of tuTOR, p-roTOR, p70S6, and p-p70S6. Simultaneously, TMP also down-regulated the expression of Bcl-2, and up-regulated that of Bax （ P 〈 0. 05 ）. Conclusion TMP inhibits the proliferation and induces the apoptosis in PC3 cells. Akt and the related downstream targets are involved in this process.

关 键 词：川芎嗪 前列腺肿瘤 肿瘤细胞 培养的 AKT 

分 类 号：R285.5[医药卫生—中药学] R730.23[医药卫生—中医学]