肾炎四味片可减轻5/6肾切除大鼠的肾脏纤维化  

Shenyansiwei Tablet alleviates renal fibrosis in 5/6 subtotal nephrectomized rats

作　　者：迟雁青[1] 林海英[1] 张涛[1] 刘茂东[1] 王保兴[1] 李英[1] 

机构地区：[1]河北医科大学第三医院肾内科,石家庄050051

出　　处：《第三军医大学学报》2013年第2期127-131,共5页Journal of Third Military Medical University

基　　金：河北省中医药管理局科研课题(2007234)~~

摘　　要：目的观察肾炎四味片对5/6肾切除大鼠肾组织核转录因子-κB(NF-κB)、转化生长因子-β1(TGF-β1)表达的影响。方法 24只Wistar大鼠分成4组:假手术组、模型组、干预组、空白对照组。肾切除术后3 d,各组予生理盐水2 mL灌胃,干预组、空白对照组加入肾炎四味片2.16 g/(kg.d)灌胃,术后第90天处死大鼠。Real-Time PCR和Westernblot分别测定各组大鼠肾脏组织中NF-κB、TGF-β1的mRNA和蛋白表达水平。免疫组化检测各组NF-κB、TGF-β1、ColⅢ的表达。结果与假手术组比较,模型组的NF-κB、TGF-β1的mRNA和蛋白水平表达增强,差异具有统计学意义(P<0.05),干预组高于假手术组(P<0.05),低于模型组(P<0.05);免疫组化结果类似。结论肾炎四味片可能通过下调NF-κB、TGF-β1的mRNA和蛋白的表达发挥延缓肾脏纤维化的作用。Objective To observed the effect of Shenyansiwei Tablet, a drug based on a prescription of traditional Chinese drugs, on the expression of NF-κB and TGF-β1 in 5/6 subtotal nephrectomized rats. Methods A total of 24 male Wistar rats were randomly divided into 4 groups, sham-operation group （group A）, subtotal nephrectomy （STN） group （ group B ）, STN with Shenyansiwei Tablet treatment group （group C）, and normal rats with Shenyansiwei Tablet treatment group （ group D）. The rats of the 2 later groups were intragastrically administrated with 2.16 g/（ kg · d）Shenyansiwei Tablet since 3 d after STN operation for 90 d. Pathological changes in the renal tissues were observed with HE, Masson and PAS staining. The expression of NF-κB, TGF-β1 and Col m in the renal tissues was detected by immunohistochemical assay. The expression levels of NF-κB and TGF-β1 in the renal tissues was measured by real-time PCR and Western blotting. Results The expression of NF-κB and TGF-β1 at protein and mRNA levels was significantly higher in group B than in group A （P 〈 0. 05）. The expression levels in group C was significantly higher than those in group A （ P 〈 0. 05 ）, but lower than in group B （P 〈 0. 05 ）. Immunohistochemical assay indicated similar results. Conclusion Shenyansiwei Tablet prevents renal fibrosis in 5/6 subtotal nephrectomized rats by down-regula- ting NF-κB and TGF-β1.

关 键 词：肾炎四味片 肾切除术 大鼠 肾纤维化 核转录因子ΚB 转化生长因子β1 

分 类 号：R285.5[医药卫生—中药学] R289.5[医药卫生—中医学]