创伤合并γ射线照射后大鼠脾脏Treg/Th17平衡的变化及其意义  

Characteristics and significance of change in Treg/Th17 balance in rats with combined trauma and γ-radiation

作　　者：蔡金玲[1] 杜丽[2] 马琼[2] 潘秀颉[2] 杨晓云[1] 肖凤君[2] 崔玉芳[2] 

机构地区：[1]中南大学基础医学院,长沙410013 [2]军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《解放军医学杂志》2013年第1期19-22,共4页Medical Journal of Chinese People's Liberation Army

基　　金：全军"十二五"医学科研重点课题(BWS11J009);国家自然科学基金(30900381)~~

摘　　要：目的观察创伤大鼠经5Gyγ射线全身照射后伤口愈合情况及脾脏Treg/Th17平衡变化的特点,探讨两者之间的关系及意义。方法清洁级雌性Wistar大鼠65只,按体重随机分为正常对照组（正常组）、单纯创伤组（单伤组）和创伤＋5Gyγ射线全身照射组（伤照组）。分别于伤照后1、3、7、14、21、28d,检测各组大鼠伤口残留面积及外周血白细胞和淋巴细胞计数,并采用流式细胞仪测定脾脏Treg细胞和Th17细胞亚群的变化。结果伤照后7-21d伤照组伤口残留面积持续大于单伤组（P〈0.01）,21d时单伤组伤口已基本愈合,而伤照组延迟至28d才基本愈合。单伤组伤照后1～7d外周血白细胞及淋巴细胞计数均明显低于正常组（P〈0.05,P〈0.01）。伤照组1~14d时外周血白细胞及淋巴细胞计数均显著低于正常组和单伤组,21~28d时白细胞计数仍显著低于正常组和单伤组（P〈0.05,P〈0.01）。伤照组大鼠脾脏Treg细胞仅在伤照后3d和7d高于正常组和单伤组（P〈0.01）,而Th17细胞于伤照后1d即显著增高（P〈0.01）,3d时升至最高,至28d时才恢复至正常组和单伤组水平。伤照组1-21d时Treg/Th17比值均显著低于正常组和单伤组（P〈0.01）,至28d时恢复至正常组和单伤组水平。结论 5Gyγ射线全身照射可导致大鼠创伤愈合延迟,Treg/Th17平衡失调在其中可能发挥了重要作用。Objective To observe the process of wound healing and investigate the characteristics of Treg/Th17 balance in rats with combined wound and 5 Gy whole-body γ-radiation（CWR） for exploring the interrelationship between wound healing and the immune balance of Treg/Th17.Methods Sixty-five female Wistar rats（clean grade） were randomly divided,depending on weight,into normal control group（normal）,wound only group（MW） and wound plus 5 Gy whole body γ-radiation group（RW）.The wound area was measured with image analyzer,the peripheral white blood cells（WBC） and lymphocytes（LYM） were counted with automatic cytometry,and the percentage of splenic regulatory T cells（Tregs） and Th17 cells was analyzed by flow cytometry at different time points post CWR.Results（1） The residual wound area was much greater in RW group than in MW group（P〈0.01） 7d to 21d after CTR,the wound surface in MW group almost healed on 21d,while that in RW group healed on 28d post CTR.（2） The peripheral WBC and LYM counts were significantly lower in MW group than in normal group（P〈0.05） 1d to 7d after CWR,and in RW group than in normal and MW group 1d to 14d after CWR.Furthermore,the WBC count was still obviously lower in RW group than in MW and normal groups 21d to 28 d after CWR.（3） The percentage of splenic Tregs was significantly higher in RW group than that in normal and MW groups（P〈0.01） at 3d and 7d after CWR,and returned to normal level at 28 d after CWR.（4） The percentage of Th17 cells was notably higher in RW group than in normal and MW groups（P〈0.01） on the first day,reached the peak value on 3 d,and returned to normal level on 28 d after CWR.（5） The ratio of Treg/Th17 was distinctly lower in RW group than in normal and MW groups from 1d to 21d,and approximated normal level on 28d after CWR,indicating that 5Gy whole-body γ-radiation could induce Treg/Th17 imbalance in wounded rats.Conclusions 5 Gy whole-body γ-radiation may delay the process of wound healing in rats.Ra

关 键 词：辐射损伤 实验性 Γ射线 伤口愈合 T淋巴细胞 调节性 TH17细胞 

分 类 号：R641[医药卫生—外科学] R818.74[医药卫生—临床医学]