非那雄胺对糖尿病小鼠肾小球微血管密度及VEGF表达的影响  

Effects of finasteride on microvascular density and VEGF expression in glomeruli of diabetic mice

作　　者：田河林[1] 康艳辉[1] 赵如同[1] 许忠新[2] 韦立顺[2] 金东岭[3] 

机构地区：[1]河北工程大学医学院药理学教研室,河北邯郸056002 [2]河北工程大学医学院解剖学教研室,河北邯郸056002 [3]河北工程大学医学院病理学教研室,河北邯郸056002

出　　处：《解放军医学杂志》2013年第1期30-33,共4页Medical Journal of Chinese People's Liberation Army

基　　金：河北省科学技术研究与发展计划(09276418D-9);河北省自然科学基金-石药集团医药联合研究基金(C2011402062)~~

摘　　要：目的探讨非那雄胺对糖尿病小鼠肾小球微血管密度(MVD)及血管内皮生长因子(VEGF)表达的影响。方法采用一次性腹腔注射链脲佐菌素150mg/kg制备糖尿病模型,将28只糖尿病小鼠随机分为4组(模型组与非那雄胺0.1、1、10mg/kg组),另取正常小鼠作为对照组(n=7)。非那雄胺组小鼠每日分别给予非那雄胺0.1、1、10mg/kg灌胃,对照组和模型组小鼠给予等量生理盐水灌胃,连续4周后取肾脏标本分别行HE染色及免疫组化染色,观察肾小球组织形态学改变及MVD、VEGF表达变化。结果模型组和非那雄胺各剂量组小鼠肾小球明显肥大,肾小球面积和体积、肾小球MVD及VEGF指数均显著大于对照组(P<0.05);与模型组比较,非那雄胺10mg/kg剂量组小鼠肾小球面积和体积、肾小球MVD及VEGF指数明显减小(P<0.05)。结论非那雄胺可抑制糖尿病小鼠肾小球VEGF表达,降低肾小球MVD。Objective To investigate the effects of finasteride on microvascular density and vascular endothelial growth factor（VEGF） expression in glomeruli of streptozotocin-induced diabetic mice.Methods Diabetes was induced in mice with a single intraperitoneal injection of streptozotocin in a dose of 150mg/kg,and they were randomly divided into 4 groups（7 each）： diabetic model group and 3 treatment groups（treated with 0.1,1,10mg/kg of finasteride,respectively）.Seven normal mice served as control group.Animals in finasteride treatment groups were intragastrically administered with finasteride 0.1,1 and 10mg/kg once daily for 4 weeks,respectively,and those in control and diabetic model group were given the same volume of normal saline at the same time.The kidney sections from all mice were stained with hematoxylin and eosin（HE） for the pathological study,and immunohistochemistry methods were performed to detect the microvascular density,and VEGF expression in glomeruli.Results Compared with control group,the glomerular area and volume,microvascular density and VEGF index were significantly increased in diabetic model and finasteride treated groups（P〈0.05）.However,the glomerular area and volume,microvessel density and VEGF index were significantly decreased in 10mg/kg finasteride treated group compared with that in diabetic model group（P〈0.05）.Conclusion Finasteride can inhibit the VEGF expression and decrease the glomerular microvascular density in diabetic mice.

关 键 词：非那雄胺 微血管密度 血管内皮生长因子 糖尿病 链脲佐菌素 

分 类 号：R961[医药卫生—药理学]