黄体酮对海水浸泡腹部开放伤大鼠胃黏膜损伤的保护作用  

Protective effects of progesterone against gastric mucosal lesion induced by open abdominal wound and seawater immersion in rats

作　　者：浦江[1] 崔立红[1] 刘超群[1] 吴姗珊[2] 李欣[1] 李辉[1] 罗哲[1] 

机构地区：[1]海军总医院消化内科,北京100048 [2]海军总医院肿瘤科,北京100048

出　　处：《解放军医学杂志》2013年第1期66-68,共3页Medical Journal of Chinese People's Liberation Army

基　　金：海军后勤部卫生部科研项目(AHJ10L002)~~

摘　　要：目的观察黄体酮对腹部开放伤合并海水浸泡大鼠胃黏膜炎性反应、黏膜组织破坏及细胞凋亡的影响。方法 30只雄性Wistar大鼠随机分为3组,每组10只。A组:腹部开放伤+灭菌注射用水皮下注射;B组:腹部开放伤海水浸泡+灭菌注射用水皮下注射;C组:腹部开放伤海水浸泡+黄体酮治疗。按16mg/kg剂量分别于浸泡后1、6、12h在皮下注射灭菌注射用水或黄体酮,16h后处死全部大鼠。计算各组大鼠胃黏膜溃疡指数(UI);光镜下观察胃黏膜病理学改变;酶联免疫法测定胃黏膜炎症介质TNF-α、IL-6含量;免疫组化检测胃黏膜组织caspase-3蛋白表达变化;TUNEL法检测胃黏膜细胞凋亡情况。结果 B组大鼠UI,胃黏膜TNF-α、IL-6水平及胃黏膜病理损伤评分均显著高于A组(P<0.01)及C组(P<0.05)。B组胃黏膜组织caspase-3蛋白表达(23.8%±3.5%)与凋亡细胞百分比(26.4%±2.8%)明显高于A组(分别为10.2%±1.6%和8.5%±1.5%,P<0.01),C组(分别为17.1%±2.3%和19.8%±2.4%)与B组比较则明显下降(P<0.05)。结论黄体酮能够有效抑制海水浸泡腹部开放伤大鼠胃黏膜炎性反应,保护胃黏膜上皮组织结构,减少胃黏膜细胞凋亡的发生。Objective To investigate the effects of progesterone on gastric mucosal inflammatory response,changes in mucosa structure,and cell apoptosis in rats with open abdominal wound and seawater immersion.Methods Thirty male Wistar rats were randomly divided into 3 groups（10 each）.The rats in group A were subjected to open abdominal wound ＋ subcutaneous injection of sterile water,and rats in group B to open abdominal wound ＋ seawater immersion ＋ subcutaneous injection of sterile water,and rats in group C to open abdominal wound ＋ seawater immersion ＋ progesterone treatment.The animal model of open abdominal wound and seawater immersion was reproduced,and the rats in groups B and C were given subcutaneous injection of sterile water or progesterone（16mg/kg） at 1,6 and 12h after immersion.All rats were executed at 16h after immersion.The ulcer index（UI） of gastric mucosa was calculated in the three groups.The pathological changes in gastric mucosa were observed under light microscope.The concentrations of tumor necrosis factor-alpha（TNF-α） and interleukin-6（IL-6） in gastric mucosa were measured by enzyme-linked immunoassay,the expression of caspase-3 in gastric mucosa was detected by immunohistochemistry,and the cell apoptosis of gastric mucosa was determined by terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling staining.Results The UI,concentrations of TNF-α and IL-6,and injury score of gastric mucosa in group B were all significantly higher than those in group A（P〈0.01） and group C（P〈0.05）.The expression of caspase-3（23.8%±3.5%） and apoptosis index（26.4%±2.8%） of the gastric mucosa in group B were higher than those in group A（10.2%±1.6% and 8.5%±1.5% respectively,P〈0.01） and group C（17.1%±2.3% and 19.8%±2.4% respectively,P〈0.05）.Conclusion Progesterone administration could suppress gastric mucosa inflammation,protect gastric mucosal structure,and reduce mucosal cell apoptosis in the rats with open abdominal wound and sea water immers

关 键 词：孕酮 胃黏膜 细胞凋亡 海水 

分 类 号：R835.1[医药卫生—航空、航天与航海医学]