假互补肽核酸及其分子生物学效应  

The Molecular Biological Effects of Pseudocomplementary Peptide Nucleic Acids

作　　者：季明辉[1] 欧青叶 顾大勇[1] 

机构地区：[1]深圳出入境检验检疫局,广东深圳518045 [2]珠海市结核病防治所,广东珠海519060

出　　处：《中国生物化学与分子生物学报》2013年第1期19-24,共6页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家自然科学基金课题(No.30972827);国家质量监督检验检疫总局科技项目(No.2009IK225)~~

摘　　要：假互补肽核酸(pseudocomplementary peptide nucleic acids,pcPNAs)是肽核酸(peptide nucleicacids,PNA)的一种衍生物,通过碱基修饰可以使pcPNAs同时与双链DNA两条链中相应的靶序列结合;而在pcPNAs识别靶序列并结合时,pcPNAs自身两条链间由于空间位阻作用,不会自身互补结合.pcPNAs与DNA、RNA甚至肽核酸相比具有独特的杂交特性,因此具有非常广泛的分子生物学效应.本文就pcPNAs寡聚体的结构、与核酸杂交的特点及杂交模式,分子生物学效应以及应用等方面进行介绍.Pseudocomplementary peptide nucleic acids（pcPNAs） are the modified derivative of peptide nucleic acids（PNA）, the pcPNAs are therefore synthesized to contain 2,6-diaminopurine and 6-thiouracil instead of adenine and thymine. Due to steric hindrance, pcPNAs are unable to form pcPNA-pcPNA duplexes but can bind to complementary DNA sequences by Watson-Crick pairing via double duplex-invasion complex formation. pcPNAs have uniqe hybridization characteristics compared with DNA / RNA, and peptide nucleic acid ,so have a very wide range of molecular biological effects. This article overviewed pcPNAs oligomeric structure, hybrid characteristics , hybrid models, molecular biological effects and applications.

关 键 词：假互补肽核酸 肽核酸 双链DNA 

分 类 号：Q617[生物学—生物物理学] Q71