干旱胁迫与复水过程中遮光对细叶小羽藓的生理生化影响  

Influence of shading during the processes of drought stress and re-watering on the physiological and biochemical characteristics of Haplocladium microphyllum

作　　者：陈文佳[1] 张楠 杭璐璐[1] 王媛[1] 季梦成[1] 

机构地区：[1]浙江农林大学风景园林与建筑学院,浙江临安311300 [2]杭州绿风园林建设集团有限公司,杭州310020

出　　处：《应用生态学报》2013年第1期57-62,共6页Chinese Journal of Applied Ecology

基　　金：国家自然科学基金项目(31070188);浙江省花卉产业创新团队项目(2009R50034);浙江省科技计划项目(2009C32066)资助

摘　　要：以细叶小羽藓为试材,分别设置全光照、遮光30%、遮光50%和遮光70%条件,采用人工控水方法研究了干旱胁迫与复水过程中细叶小羽藓的质膜透性、渗透调节能力及抗氧化酶系统的变化.结果表明:干旱胁迫过程中,细叶小羽藓的相对含水量呈下降趋势,而相对电导率在干旱21d时达到最大值,且全光照下的相对电导率最高(58.0%);可溶性糖和游离脯氨酸含量均先上升后下降;超氧化物歧化酶(SOD)和过氧化物酶(POD)活性先持续上升而后下降.在复水过程中,细叶小羽藓的相对含水量与干旱胁迫时相反;相对电导率、可溶性糖含量、游离脯氨酸含量、SOD和POD活性先小幅回升后逐渐下降,但SOD和POD活性仍较高;复水后大部分指标都恢复到干旱胁迫前水平.细叶小羽藓具备复苏植物的典型特征,在变水过程中有较强的抗旱能力.An artificial water control experiment was conducted to study the influence of shading on the membrane permeability, osmoregulation capability, and protective enzyme activities of Haplocladium microphyllum during the processes of drought stress and re-watering. Four treatments of shading were installed, i. e. , full daylight and 30%, 50% and 70% of shading. During drought stress, the relative water content of 11. microphyllum presented a decreasing trend, while the relative conductivity reached the maximum when the drought stress lasted for 21 days, and the conductivity was high up to 58.0% under full daylight. The soluble sugar and free proline contents and the SOD and POD activities of the plant under drought stress were decreased after a sustained increase. During re-watering, the relative Water content of 11. microphyllum showed an increasing trend, the relative conductivity, soluble sugar and free proline contents, and SOD and POD activities had a slight increase first and decreased gradually then, but the SOD and POD still kept high activities. After rewatering, most of the test indices recovered to the levels before the drought stress. It was suggested that 1t. microphyllum had the typical characteristics of resurrection plants, possessing a strong capability against drought during the variation of soil moisture content.

关 键 词：细叶小羽藓 水分胁迫 渗透调节 抗氧化酶系统 

分 类 号：Q945[生物学—植物学]