东莨菪内酯与双脱甲氧基姜黄素对朱砂叶螨致死效应模拟  

Lethal effects of scopoletin and bisdemethoxycurcumin against Tetranychus cinnabarinus Boisd(Acari:Tetranychidae):A simulation study

作　　者：杨振国[1] 张永强[1] 丁伟[1] 罗金香[1] 

机构地区：[1]西南大学植物保护学院,重庆400716

出　　处：《应用生态学报》2013年第1期197-204,共8页Chinese Journal of Applied Ecology

基　　金：科技部农业科技成果转化基金项目(2010GB2F100388);教育部博士点新教师基金项目(20100182120021)资助

摘　　要：采用玻片浸渍法测定了植物源杀螨活性物质东莨菪内酯、双脱甲氧基姜黄素及两者联合作用最佳增效配比(东:双=7:6)对朱砂叶螨雌成螨的致死效应,并采用时间-剂量-死亡率(TDM)模型进行模拟.结果表明:所建TDM模型均通过Hosmer-Lemoshow拟合异质性检验,朱砂叶螨雌成螨对3种药剂浓度变化的敏感程度为东莨菪内酯>两者最佳配比>双脱甲氧基姜黄素,双脱甲氧基姜黄素、东莨菪内酯和两者最佳配比对朱砂叶螨雌成螨的致死率高峰期分别为处理后32、28和32h;处理后48h的LC50和LC90分别为0.3324、0.2035、0.2195mg·mL-1和2.1198、0.9521、1.1617mg·mL-1;浓度为1.0和2.0mg·mL-1处理下,LT50分别为7.4、6.0、6.1h和6.4、4.8、5.0h.因此,东莨菪内酯与双脱甲氧基姜黄素最佳配比和东莨菪内酯具有相近的杀螨动态及时间-剂量效应,表现出较好的杀螨活性和增效作用,具有一定的开发利用价值.By using slide-dip bioassay method under laboratory condition, an investigation was made on the lethal effects of scopoletin, bisdemethoxycurcumin （ BDMC）, and their combination at an optimal mass ratio （7：6） of scopoletin to BDMC against the adult females of Tetranychus cinnabarinus Boisd. A time-dose mortality model （TDM） was established, which passed the Hosmer- Lemeshow test. The sensitivity of the adult females to the concentration change of the acaricldes was in the sequence of seopoletin 〉 optimal mass ratio of scopoletin to BDMC 〉 BDMC. The peak mortality of the female adults was found at 32, 28 and 32 h after treated with BDMC, seopoletin, and their combination at the optimal mass ratio, respectively. The values of the LCs0 and LC9o at 48 h after treated with BDMC, seopoletin, and their combination at the optimal mass ratio were 0. 3324, 0.2035 and 0.2195 mg·mL^-1, and 2. 1198, 0.9521 and 1. 1617 mg ·mL^-1 ,and the median lethal time （ LT50 ） of BDMC, scopoletin, and their combination at the optimal mass ratio was 7.4, 6.0 and 6.1 h at the concentration 1.0 mg·mL^-1 , and 6.4, 4.8 and 5.0 h at the concentration 2.0 mg ·mL^-1 , respectively. The acaricidal activity and time-dose response of the optimal combi- nation of scopoletin and BDMC were closer to those of seopoletin, suggesting a synergistic acaricidal activity of the combination of scopoletin and BDMC, which was worthy to be developed for application.

关 键 词：朱砂叶螨 黄花蒿 姜黄 时间-剂量-死亡率模型 杀螨活性 

分 类 号：S482.52[农业科学—农药学]