生长微环境下原位矿化磷酸钙诱导的细胞死亡(英文)  

Cell Death Induced by In Situ Mineralized Calcium Phosphate in Growth Microenvironment

作　　者：刘朋[1] 陈燕[1] 王广川[1] 王本[1] 徐旭荣[1] 唐睿康[1] 

机构地区：[1]浙江大学理学院化学系,杭州310027

出　　处：《无机化学学报》2013年第1期89-94,共6页Chinese Journal of Inorganic Chemistry

基　　金：国家自然科学基金(No.91127003)资助项目

摘　　要：本文主要研究了不同量的磷酸钙在生长环境下对骨肉瘤细胞死亡的影响。通过在无钙培养基中加入氯化钙原位合成得到所需磷酸钙颗粒,然后用无钙磷培养基按照不同的比例稀释所得磷酸钙颗粒。MTT结果表明,24 h后的细胞凋亡与培养基中磷酸钙的量有关。当浓度超过240μg.mL-1时,细胞出现大量死亡。细胞超薄切片结果也表明,当磷酸钙浓度超过240μg.mL-1时,可以观察到有大量磷酸钙存在于细胞内;而磷酸钙浓度低于240μg.mL-1时,细胞内观察不到磷酸钙的存在。这些结果表明:240μg.mL-1对于细胞死亡是一个非常重要的转折点,可能超出了细胞的自我修复范围或者承受能力。这些结果对于磷酸钙在生物医学工程,如组织工程、药物输送、基因转染等方面具有一定的指导意义。The correlation of calcium phosphate quantity and their lethal effect on human osteosarcoma cells in growth microenvironment was investigated. The calcium phosphate nanoparticles （CaP-NPS） were in situ generated by adding calcium solution to cell culture media. The media with different amounts of CaP-NPS were prepared by suspending the obtained CaP-NPS into cell culture solutions. The viability of human osteosarcoma cells cultured in the media was examined by MTI＇ method. We found that CaP-NPS induced death of the co- cultured cells depending on their amounts. MTT assay revealed that CaP-NPS could obviously reduce the cell viability by inducing cell death when their quantity was above the threshold 240μzg.mL^-1. The transmission electron microscopy （TEM） results revealed that lots of CaP-NPS were observed in cytoplasm of cocultured cells when the concentration of CaP-NPS was 〉240 μg. mL^-1. In contrast, no CaP-NPS was found within the cocuhured cells when the concentration of CaP-NPS was 〈240 μg.mL^-1. Our results implied that 240 μg. mL^-1 CaP-NPS was a critical point for cell viability, exceeding which would cause severely cell death because it exceeded the limit of cell self-clarify or tolerant ability. This study may provide a guide for the CaP-NPS applications in medical engineering such as tissue engineering, drug delivery, and gene transfection.

关 键 词：生物矿化 磷酸钙 细胞死亡 纳米颗粒 临界浓度 

分 类 号：O611.6[理学—无机化学]