丝胶对糖尿病大鼠脊髓及脊神经节细胞神经肽Y表达的作用  

Changes of neuropeptide Y expression in spinal cord and spinal ganglia cells of diabetes mellitus rats and protective effects of sericine

作　　者：李强[1] 李山山[2] 张艳[1] 钟美蓉[1] 陈志宏[1] 

机构地区：[1]承德医学院人体解剖学教研室,河北承德067000 [2]天津医科大学附属肿瘤医院肝胆科

出　　处：《中国老年学杂志》2013年第1期110-111,共2页Chinese Journal of Gerontology

基　　金：河北省科技厅项目(No.08276101D-9)

摘　　要：目的探讨2型糖尿病大鼠脊髓及脊神经节细胞神经肽Y(NPY)蛋白表达的变化及丝胶的保护作用。方法选用雄性SD大鼠36只,随机分为正常对照组、2型糖尿病模型组和丝胶治疗组(n=12)。采用免疫组化SP法观察坐骨神经对应的脊髓节段(L4-6)和脊神经节神经细胞NPY蛋白的表达。结果 NPY蛋白免疫阳性产物为棕褐色,呈颗粒状,主要分布于脊髓前角细胞和脊神经节细胞的胞浆和胞核,以胞浆为主。模型组大鼠脊髓前角细胞和脊神经节细胞NPY蛋白的表达明显高于正常对照组,丝胶治疗组大鼠脊髓前角细胞和脊神经节细胞NPY蛋白的表达明显低于模型组(P<0.01)。结论糖尿病模型大鼠脊髓前角细胞和脊神经节细胞NPY蛋白的表达明显上调;丝胶可通过下调NPY蛋白的表达,保护糖尿病坐骨神经相关神经元胞体。Objective To investigate the changes of neuropeptide Y （NPY） expression in spinal cord and spinal ganglia cells of type 2 diabetes mellitus （T2DM） rats and protective effects of sericine. Methods 36 male SD rats were randomly divided into （n = 12） ： normal control, T2DM model and sericine treatment groups. SP immunohistochemical staining was used to observe the expression of NPY protein in spinal cord anterior horn ceils and spinal ganglia cells. Results NPY immunopositive products were tan granules, mainly distrib-uted at the cytoplasm and nucleus, especially at cytoplasm. The NPY expression in anterior horn cells of spinal cord and spinal ganglia cells of rats in model group were obviously higher than those of rats in normal control group （P 〈 0. 01 ）. The NPY expression in anterior horn ceils of spinal cord and spinal ganglia cells of rats in sericine treatment group were obviously lower than those of rats in model group （P 〈 0. 01 ）. Conclusions The NPY expression in spinal cord anterior horn cells and spinal ganglia cells is up-regulated obviously. Sericine can protect sciatic nerve correlated neuronal soma by down-regulating NPY expression.

关 键 词：丝胶 糖尿病 脊髓 脊神经节 NPY 

分 类 号：R587[医药卫生—内分泌]