枯草芽孢杆菌B-903菌株的研究Ⅲ.影响抗菌物质产生和积累的主要因素  

Study on Bacillus subtilis B 903 Strain Ⅲ.The Factors Affect on the Production of Antifungal Substance

作　　者：孔建[1] 王文夕[1] 赵白鸽[1] 申效诚[1] 

机构地区：[1]河南省农业科学院植物保护研究所,郑州450002

出　　处：《中国生物防治》2000年第2期65-68,共4页Chinese Journal of Biological Control

基　　金：河南省农科院基础研究项目

摘　　要：培养液的初始 p H值直接影响 B- 90 3菌株的生长繁殖 ,从而对其抗菌物质的产生和积累产生重要影响。 p H值小于 5时 ,不利于细菌生长繁殖 ,滤液中抗菌物质活性弱 ;p H值在 6～ 8时有利于细菌的增殖 ,抗菌活性亦最强。培养过程中供氧条件与菌体繁殖和抑菌活性呈正相关 ,而培养液初始接菌量高时可缩短抗菌物质出现的时间。基本营养物质的初步筛选试验证明 :大米粉、豆饼粉、麦麸、酵母粉和 Ca CO3 及 KH2 PO4是发酵培养基中最佳的营养物质 ,可满足抗菌物质产生对碳、氮和矿物质的营养需求。B- 90 3的发酵过程可分为两个阶段 :在 30℃下 ,2 4小时内 ,细菌数量达最大值 ,p H值由低向高变化 ,但此阶段检测不出抗菌物质的活性 ;第二阶段 ,培养 41小时后 ,抗菌物质活性开始显露 ,至 112小时后达最大值 ,p H值也逐渐由高变低。培养过程中 ,pThe influence of pH, oxygen, inoculum level and nutrition on bacterial growth and production of antifungal substance was examined. The initial optimum pH of liquid culture broth for bacterial growth and antifungal production was 6-8. Oxygen enhanced the growth of bacteria and antifungal substance production. The high inoculum levels increased the bacterial growth and shorten the time for antifungal substance production. The powder of rice, soybean cake, yeast, and wheat bran, CaCO 3 and KH 2PO 4 were better nutrients for the bacteria growth and antifungal substance production. There were two stages during the fermentation process at 30℃. The growth of bacteria increased at the first 24 h and the pH of broth rose, but no antifungal activity was observed until 41 h later and reached to the highest level at 114 h. The pH of broth declined at this stage.

关 键 词：枯草芽孢杆菌 抗菌素 培养条件 营养物质 PH值 

分 类 号：S476.11[农业科学—农业昆虫与害虫防治] Q939.124[农业科学—植物保护]