加味四逆散对慢性身心应激模型大鼠胃粘膜超微结构及脑肠轴的影响  

Effects of Jiaweisinisan on gastric mucosal ultrastructure and brain-gut axis in a rat model of chronic psychological stress

作　　者：谢慧臣[1,2] 刘芬[1,2] 杨强[3] 熊常初[1] 

机构地区：[1]湖北中医药大学,湖北武汉430065 [2]湖北民族学院医学院中医教研室,湖北恩施445000 [3]湖北民族学院医学院附属医院中医部,湖北恩施445000

出　　处：《南方医科大学学报》2013年第1期103-107,共5页Journal of Southern Medical University

基　　金：湖北省教育厅科学研究计划重大项目(Z20092901);湖北民族学院博士科研启动基金(20110112)

摘　　要：目的建立大鼠慢性身心应激模型,探讨加味四逆散对模型大鼠胃粘膜超微结构、血浆促肾上腺皮质激素(ACTH)和皮质醇(CORT)含量、胃组织胃泌素受体(GASR)、空肠组织血管活性肠肽Ⅱ型受体(VIPR2)mRNA表达的影响,阐明加味四逆散干预应激性胃肠功能障碍的机制。方法大鼠随机分为正常组,模型组,加味四逆散大、中、小剂量组,奥美拉唑组,采用慢性身心应激方法建立大鼠应激模型,经加味四逆散等干预后透射电镜法观察腺胃区胃粘膜组织细胞及细胞间连接的超微结构改变,放免法测定血浆ACTH和血清CORT的变化,并用RT-PCR法检测胃组织GASR、空肠组织VIPR2 mRNA表达变化。结果电镜观察显示正常组大鼠胃粘膜上皮细胞形态及大小正常;模型组大鼠胃粘膜上皮细胞细胞器及胞核受损严重;其余治疗组均较模型组不同程度好转。与模型组比较,加味四逆散方各给药组、奥美拉唑组大鼠血中ACTH和CORT含量不同程度降低;而胃组织GASR mRNA表达均升高,空肠组织VIPR2 mRNA表达则降低,差异有统计学意义(P<0.05或P<0.01)。结论加味四逆散可显著改善慢性身心应激模型大鼠胃粘膜组织细胞的微观病理形态,调节脑肠轴状态并可改善胃组织GASR、空肠组织VIPR2 mRNA表达。Objective To study the effect of Jiaweisinisan （JWSNS）, a traditional Chinese herbal medicinal recipe, on gastric mucosal ultrastructure and brain-gut axis in rat models of chronic psychological stress and elucidate the mechanism of ]WSNS for ameliorating stress-induced gastrointestinal dysfunction. Methods Sixty rats were randomly assigned into normal control group, model group, 3 JWSNS groups （high, moderate, and small doses）, and omeprazole group （n=10）. Rat models of chronic psychological stress were established by random stressful stimulations, and following the corresponding interventions, plasma adrenocorticotropic hormone （ACTH） and cortisol （CORT） levels were detected using radioimmunoassay, and the mRNA expressions of gastrin receptor in the gastric tissue （GASR） and vasoactive intestinal peptide II receptor （VIPR2） in the jejunal tissue were examined using RT-PCR. Transmission electron microscopy was employed to examine the ultrastructural changes in the gastric mucosa tissue cells of the glandular stomach area and alterations in the intercellular junctions. Results Electron microscopy revealed obvious damages in gastric mucosal epithelial cell organelles and nuclei in the model rats. These damages were ameliorated after treatments with JWSNS and omeprazole. Compared with the model group, the 3 JWSNS groups and omeprazole group all showed significantly lowered plasma ACTH and CORT levels, increased gastrin receptor mRNA expression and decreased jeiunal VIPR2 mRNA expression （P〈0.05 or 0.01）. Conclusion ]WSNS can obviously ameliorate the pathologies of the gastric mucosa cells, regulate the state of brain-gut axis, and modulate the gastric gastrin receptor and jejunal VIPR2 mRNA expressions in rats with chronic psychological stress.

关 键 词：加味四逆散 应激 胃粘膜 脑肠轴 促肾上腺皮质激素 皮质醇 胃泌素受体 血管活性肠肽Ⅱ型受体 

分 类 号：R285.5[医药卫生—中药学]