MAP-2和巢蛋白在人胚胎舌肌组织发育过程中的表达  

Expressions of microtubule-associated protein 2 and nestin in the development of human embryo and fetal tongue muscles

作　　者：刘学红[1] 张泳[2] 

机构地区：[1]绍兴文理学院医学院组织学与胚胎学教研室,浙江绍兴312000 [2]绍兴文理学院医学院医学实验中心,浙江绍兴312000

出　　处：《南方医科大学学报》2013年第1期135-137,共3页Journal of Southern Medical University

基　　金：浙江省教育厅科研计划项目(Y201018535)

摘　　要：目的探讨微管相关蛋白2(MAP-2)和巢蛋白与人胚胎舌肌组织的发育关系。方法应用免疫组织化学PV法检测第2-4三个月龄段,共16例人胚胎,舌肌组织内MAP-2和巢蛋白的表达规律。结果第2-4个月龄段,MAP-2和巢蛋白在人胚胎舌肌组织内均呈阳性表达。第2个月龄段未见明显MAP-2阳性表达细胞,第3-4个月MAP-2在舌肌内阳性表达细胞数依次为24.14±8.28、15.86±3.89,阳性表达强度依次为109.42±11.62、124.27±8.73。第2-4个月巢蛋白在舌肌组织内阳性细胞数量依次为12.50±3.17、19.00±7.63、22.80±6.91,阳性细胞表达强度依次为为119.99±24.02、102.20±11.76、98.24±10.66。随着胎龄增大,MAP-2在肌组织内阳性细胞表达数量呈先增高再降低趋势,阳性细胞表达强度呈逐渐增高趋势;巢蛋白在肌组织内阳性细胞表达数量呈逐渐增高趋势,阳性细胞表达强度呈逐渐降低趋势。结论 MAP-2和巢蛋白参与调节人胚胎舌肌组织的发育过程。Objective To explore the role of microtubule-associated protein 2 （MAP-2） and nestin in the development of tongue muscles of human embryos and fetuses. Methods PV immunohistochemistry was used to detect the expressions of MAP-2 and nestin proteins in the tongue tissues of human embryos and fetuses at the second, third and fourth months of gestation. Results MAP-2 and nestin positivity was detected in the tongue muscles of human embryos at 2 to 4 months of gestation. In the embryos at the second month of gestation, no obvious MAP-2 positive cells were found in the tongue muscles; at 3 and 4 months, the number of MAP-2-positive cells in the tongue muscles was 24.14±8.28 and 15.86±3.89, with the expression intensity of 109.42±11.62 and 124.27±8.73, respectively. At 2, 3 and 4 months of gestation, the number of nestin-positive cells in the tongue muscles was 12.50±3.17, 19.00±7.63, and 22.80±6.91, with expression intensity of 119.99±24.02, 102.20±11.76, and 98.24±10.66, respectively. As the gestational age increased, the number of MAP-2-positive cell number continued to decline following a transient increase but the expression intensity kept increasing; nestin-positive cells increased continuously but the expression intensity kept decreasing in the embryonic or fetal tongue muscles. Conclusion MAP-2 and nestin proteins are involved in the regulation of the development of tongue muscles in human embryos and fetuses.

关 键 词：舌肌 微管相关蛋白2 巢蛋白 胚胎 

分 类 号：R321[医药卫生—人体解剖和组织胚胎学]