金雀异黄素通过调控miR-27a及其靶基因对卵巢癌细胞SKOV3生长的影响  

Effects of genistein on the growth of ovarian cancer cell SKOV3 by regulating miR-27a and target gene expression

作　　者：徐琳琳[1] 孙庆敏[2] 罗璇[1] 翟溯澜[1] 李萍[1] 赵小梅[1] 王雪融[1] 

机构地区：[1]南京医科大学基础医学院药理学系 [2]南京医科大学附属无锡市妇幼保健院药剂科

出　　处：《中国临床药理学与治疗学》2012年第12期1321-1326,共6页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：国家自然科学基金项目(81001444;30873099;81102458;81172004)

摘　　要：目的:研究金雀异黄素对卵巢癌细胞株SKOV3生长的抑制作用及其可能的机制。方法:收集卵巢癌组织及良性组织,应用RT-PCR法检测miR-27a的表达。用不同浓度的金雀异黄素处理细胞,通过SRB法检测金雀异黄素单用或加入miR-27amimics后对细胞株生长的抑制作用;经RT-PCR和Western blot检测miR-27a及靶基因Sprouty2表达的变化。结果:miR-27a在卵巢癌组织中的表达高于良性组织(P<0.05)。金雀异黄素可呈浓度和时间依赖的方式抑制卵巢癌细胞SKOV3生长;金雀异黄素浓度依赖性地下调miR-27a,上调Sprouty2的表达,且miR-27amimics能够部分拮抗金雀异黄素抑制细胞生长的作用。结论:金雀异黄素可能通过下调致癌miR-27a的表达,发挥抑制卵巢癌细胞生长的作用。AIM： To investigate the effects of genistein on the growth of ovarian cancer cell SKOV3 and potential mechanism.METHODS： The expression of the miR-27a in formalin-fixed paraffin-embedded（FFPE） ovarian cancer tissues and benign ovarian tissues were examined by RT-PCR.SKOV3 cells were treated with different concentration of genistein.Then the SRB assays were performed to assess the growth inhibition of genistein to ovarian cancer cells.RT-PCR and Western blot were used to test expression of miR-27a and Sprouty2 respectively.RESULTS：The expression levels of miR-27a in FFPE ovarian cancer tissues were significantly higher than those seen in benign ovarian tissues.Genistein could markedly decrease human ovarian cancer cell growth in time-and dose-dependent manner and downregulate the expression of miR-27a,which was accompanied by significantly increased expression of Sprouty2.CONCLUSION： Genistein has an inhibitory effect on the growth of ovarian cancer cells,which was due to the downregulation of miR-27a expression.

关 键 词：金雀异黄素 卵巢癌 生长 miR-27a Sprouty2 

分 类 号：R965.1[医药卫生—药理学]