丁酸钠对庆大霉素耳聋保护的体内研究  

Sodium Butyrate Reduces Gentamicin-Induced Hearing Loss

作　　者：王洁[1] 王亚菲[1] 温立婷[1] 王烨[1] 陈福权[1] 邱建华[1] 

机构地区：[1]第四军医大学西京医院耳鼻咽喉头颈外科,西安710032

出　　处：《中华耳科学杂志》2012年第4期489-492,共4页Chinese Journal of Otology

基　　金：西京医院学科助推计划(XJZT09M14);国家自然科学基金青年项目(81100713)资助

摘　　要：目的通过动物实验验证组蛋白去乙酰化酶抑制剂丁酸钠是否可以保护耳蜗毛细胞免受庆大霉素引起的耳毒性损害。方法 10只听力正常豚鼠被用于本实验。向每只动物右耳的圆窗龛放置小块明胶海绵(0.3mm3)并注入10μl丁酸钠(100mg/ml)溶液,左耳圆窗龛内置放的明胶海绵则注入10μl人工外淋巴液作为对照。手术后第三天开始按照每公斤体重200mg的剂量每天一次性对豚鼠肌肉注射庆大霉素,连续用药5天。在停药后第3天检测听性脑干诱发电位,常规制备全耳蜗基底膜铺片结合鬼笔环肽染色,对全耳蜗毛细胞进行计数并制备全耳蜗毛细胞图。结果圆窗龛置放丁酸钠溶液组的听性脑干诱发电位阈值比圆窗龛置放外淋巴液组低,两组资料相比有显著性统计学差异(P<0.01),圆窗龛置放丁酸钠溶液组的耳蜗毛细胞缺失程度比圆窗龛置放外淋巴液组轻。结论组蛋白去乙酰化酶抑制剂丁酸钠对庆大霉素导致的豚鼠耳聋和耳蜗损害具有一定的保护作用。Objective To investigate the protective effects of histone deacetylase （HDAC） inhibitor sodium butyrate （NAB） against gentamicin （GM） ototoxicity in vivo. Methods Ten guinea pigs with normal hearing response were used for this study. A small piece of gelatin sponge （0.3 mm3） was surgically placed in the round window niche in both ears. NaB （100 mg/ml, 10μl） was infused into the gelatin in the right round window, whereas artificial perilymph （10μl）was infused into the gelatin in the left round window niche as control. Three days after surgery, animals received intramuscular injection of gentamicin （200mg/kg/day） for 5 consecutive days. The threshold of Auditory Brainstem Responses （ABRs） was measured 3 days after the final GM injection. Cochlear surface preparations were made with palloidin staining to determine the num- ber of missing cochlear hair cells as a pattern of cochleogram. Results Compared to the left ear that was treated with artifi- cial perilymph, lower thresholds of ABR and less hair eell loss were seen in NaB treated ears. Conclusion NaB may allevi- ate gentamicin-induced cochlear lesions.

关 键 词：丁酸钠 庆大霉素 耳聋 组蛋白去乙酰化酶抑制剂 

分 类 号：R764.43[医药卫生—耳鼻咽喉科]