RANTES在子宫内膜异位症在位内膜及异位内膜的表达及其意义  

Expression and Significance of RANTES in Ectopic and Eutopic Endometrium of Patients with Endometriosis

作　　者：范小斌 曹引丽 蔺小贤 徐叶红 

机构地区：[1]陕西省妇幼保健院,陕西西安710003

出　　处：《实用妇产科杂志》2012年第12期1035-1038,共4页Journal of Practical Obstetrics and Gynecology

基　　金：陕西省科技计划项目(编号:2009JM4031)

摘　　要：目的:探讨调节活化正常T细胞表达与分泌的趋化因子(RANTES)在子宫内膜异位症在位内膜及异位内膜的表达及在子宫内膜异位症发病中的作用。方法:以2010年1～10月因子宫内膜异位症在我院行手术治疗的30例患者的在位内膜及异位内膜为研究组,以同期在我院就诊取IUD的30例患者的正常子宫内膜作为对照组,检测3组标本中RANTESmRNA表达及蛋白水平。并检测3组培养上清液对单核细胞趋化活性的影响。结果:①IL-1β刺激48小时,异位内膜组细胞RANTESmRNA表达及蛋白水平均增加,与在位内膜组和正常内膜组细胞比较,差异均有统计学意义(P<0.05)。IL-1β刺激72小时,异位内膜组、在位内膜组细胞RANTESmRNA表达及蛋白水平均增加,异位内膜组细胞与在位内膜组和正常内膜组细胞比较,差异均有统计学意义(P<0.05);在位内膜组细胞与正常内膜组细胞比较,差异也有统计学意义(P<0.05)。②异位内膜组及在位内膜组单核细胞趋化指数明显高于正常内膜组(P<0.05)。结论:子宫内膜异位症患者的在位内膜及异位内膜RANTESmRNA表达及蛋白水平均增加。RANTES通过促进单核细胞的趋化活性,可能在子宫内膜异位症的发病中发挥作用。Objective：T0 investigate the expression of the factor of regulated upon activation normal T cell （RANTES） in ectopic and eutopic endometrium of patients with endometriosis and its relevance with patho- genesis of endometriosis. Methods： A total of 30 patients with endometriosis who were hospitalized in Shanxi province maternal and children hospital from Jan 2010 to Oct 2010, were recruited as study group, in which ectopic and eutopic endometrium were divided into two subgroups. In the meantime,30 patients with normal endometrium were chosen as control group. The expressions of RANTES mRNA and protein in endometrium were measured by PCR and ELISA,respectively,and the influence of cultural supernatants to the monocyte chemoattract activity in the three groups were also investigated. Results： ① The expressions of RANTES mRNA and protein in ectopic endometrium were higher than those in eutopic and normal endometrium sitimu- lated by IL-113 after 48 hours（ P 〈0.05）. After 72 hours,The expressions of RANTES mRNA and protein in eutopic endometrium showed statistically higher than those in normal endometrium （ P 〈 0.05）. ②The chemo- tactic index of monocyte cell going through matrigel membrane in ectopic and eutopic endometrium groups were markedly higher than that in normal endometrium （P 〈 0. 05）. Conclusions：The higher expression of RANTES in eutopic and ectopic endometrium and its influence to the chemtaxis of monocyte may be involved in the pathogenesis of endometriosis.

关 键 词：子宫内膜异位症 调节活化正常T细胞表达与分泌的趋化因子 在位内膜 异位内膜 

分 类 号：R711.71[医药卫生—妇产科学]