黄芩、板蓝根等清热解毒药对流感病毒所致肺炎小鼠炎性因子蛋白表达的影响  

The influence of representative herbs of clearing and detoxifying drugs effect on inflammatorycytokines expression of mice lung homogenate infected by influenza virus FM1

作　　者：王成祥[1] 曹鸿云 程淼[1] 徐红日[1] 王明哲[1] 徐丽丽[1] 于会勇[1] 李倩[1] 

机构地区：[1]北京中医药大学东直门医院,100700 [2]北京市王府医院

出　　处：《中华实验和临床病毒学杂志》2012年第6期415-418,共4页Chinese Journal of Experimental and Clinical Virology

基　　金：国家自然科学基金（30873254）;教育部博士学科点专项科研基金资助项目（20070026012）

摘　　要：目的研究清热解毒代表药物对流感病毒FMl感染肺炎小鼠肺匀浆炎性细胞因子的影响，以筛选出对流感病毒具有更好免疫调控作用的清热解毒中药。方法建立流感病毒鼠肺适应株（FMl）感染的小鼠肺炎模型，采用双抗夹心ELISA法分别在感染后第1、3、5、7天检测空白对照组、模型对照组、黄芩组、板蓝根组、白头翁组、虎杖组、白花蛇舌草组小鼠肺匀浆TNF-α、IFN-r、IL－6、IL-1、IL-10含量的变化。结果流感病毒感染小鼠后，模型组TNF-13t，IL-1，IL－6，IFN－1的蛋白表达高于正常空白组，在第3天达峰值，差异有统计学意义（P〈0．05）。清热解毒代表药物黄芩、板蓝根均可以降低TNF-α、IL-6、IL－1含量，提高IL-10、IFN-r的含量，第3、5天时作用较明显，有统计学意义（P〈0．05）。白头翁、虎杖及白花蛇舌草对炎性细胞因子在一定程度上有调节作用，但效果不及黄芩和板蓝根。结论清热解毒代表药物黄芩、板蓝根可通过调节细胞因子水平来干预流感病毒引起的免疫损伤，从而发挥抗流感病毒的作用。Objective In order to screen out a certain kind of traditional medicine which has a better role in immune regulatory, the influence of representatives of heat clearing and detoxicating herb on inflammatory cytokines protein expression of mice lung homogenate infected by FM1 have been observed. Method Modeling mice infected by FM1. On the first, third, fifth and seventh day after FM1 infection, tumor necrosis factor-a（TNF-alpha） ,interleukin-1 （ IL-I ） , interleukin-6 （IL-6） ,interleukin-10（IL-10） ,and r-interferon （IFN-r） expression in mice lung homogenate of normal control group, model control group, scutellari group, isatidis group, pulsatilla group, polygonum cuspidatum group and oldenlandia group have been tested by ELISA method. Result The expression of TNF-α, IL-6, IFN-r and IL-10 in mice lung homogenate reaches its peak on the third day after FM1 infection, significantly higher than the control group （ P 〈 0.05 ）. Scutellari and isatidis are two representatives of heat clearing and detoxicating herb, which can decrease the expression of TNF-α, IL-6 and IL-1 and increase the expression of IL-IO,IFN-r/. The effect are more pronounced and statistically significant （ P 〈 0.05 ） on the third and fifth day after infection, pulsatilla, polygonum cuspidatum and oldenlandia can also regulate the inflammatory cytokines,but the effect are not so obvious as scutellari and isatidis. Conclusion Scutellari and isatidis, two representatives of heat clearing and detoxicating herb, have a good intervention on immune damage caused by influenza virus through adjusting the balance of inflammatory cytokines and anti-inflammatory cytokines.

关 键 词：中草药 流感病毒A型 细胞因子类 酶联免疫吸附测定 

分 类 号：R28[医药卫生—中药学]