膜蛋白Flotillin-1与细胞膜PrPC内吞相关性研究  

Study on the correlation between membrane protein Flotillin-1 and PrPc Endocytosis

作　　者：任科[1] 王轲[1] 许尹[1] 李龙珠[1] 张瑾[1] 王荟[1] 燕玉娥[1] 董小平[1] 高晨[1] 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所传染病预防控制国家重点实验室,北京100052

出　　处：《中华实验和临床病毒学杂志》2012年第6期435-438,共4页Chinese Journal of Experimental and Clinical Virology

基　　金：国家自然科学基金青年基金（31100117）;传染病预防控制国家重点实验室青年基金（2012SKLID201）

摘　　要：目的研究膜蛋白Flotillin-1是否与PrP。蛋白内吞相关。方法利用免疫印迹法观察不同细胞株中Flotillin-1表达情况；利用免疫共沉淀的方法，检测Flotillin-1和PrPc蛋白在Cu2＋存在条件下相互作用的情况。结果①免疫印迹法显示Flotillin-1在多种细胞株中均有表达，且表达量没有明显差别；②免疫共沉淀实验（IP）结果提示在cu2＋处理条件下细胞中有PrPc与Flotillin-1蛋白复合体的形成，并且与cu2＋浓度以及cu2＋处理时间相关；结论膜蛋白Flotillin-1与细胞膜PrPc内吞过程存在相关性。Objective To explore whether the membrane-associated protein Flotillin-1 has relationship with endocytosis of PrPc. Methods The expression of Flotillin-1 in different cell lines was detected with the method of Western Blot; the interaction between Flotillin-1 and PrPc in Cells which were treated with copper ions was observed using immunoprecipitation method. Results ①Flotillin-1 was widely expressed in many cell lines without significant difference in the amounts of expression level; ②Only in the appearance of copper ions, the protein complexes of PrPc and Flotillin-1 can be detected with the method of IP, which were related to copper ions concentration and processing time. Conclusion The membrane- associated protein Flotillin-1 has the relationship with the endocytosis of PrPc.

关 键 词：朊病毒 病毒基质蛋白质类 胞吞作用 铜 

分 类 号：R392[医药卫生—免疫学]