唑来膦酸与γδTCR单抗体外扩增HIV感染者Vδ2T细胞的效果比较  

The comparison between HIV-infected patients＇ Vdelta2 T cells expansion efficiencies by zoledronicacid and gammadelta TCR monoclonal antibody in vitro

作　　者：许文[1] 武晓丽[1] 谢杨新[1] 杨俊连[1] 聂为民[1] 赵敏[1] 陈威巍[1] 

机构地区：[1]解放军第三〇二医院感染性疾病诊疗与研究中心,北京100039

出　　处：《中华实验和临床病毒学杂志》2012年第6期442-445,共4页Chinese Journal of Experimental and Clinical Virology

基　　金：国家自然科学基金资助项目（81072423）

摘　　要：目的观察唑来膦酸和rδTCR单抗两种诱导方法对HIV感染者的V82T细胞的扩增效果，探讨效率更高的扩增方法。方法选择65例HIV感染者和38例健康对照者，应用唑来膦酸法和rδTCR单抗法分别诱导其外周血单个核细胞（PBMC），分别在培养的0d、7d、14d应用流式细胞术检测其rδT细胞及其亚群的百分率及Vδ2T细胞增殖情况，比较两种方法的诱导效果。结果外周血中HIV感染者Vδ2T细胞的数量和比例与健康对照相比显著下降；扩增14d后，rδTCR单抗法诱导HIV感染者和健康对照者的V82T细胞占CD3＋细胞的百分比分别为（17．6±19．8）％和（64．3±4．5）％，V82T细胞扩增倍数分别为54±40和74±29；而采用唑来膦酸法的诱导结果分别为（69．6±21．2）％和（97．3±1．7）％以及538±11和5984±721。结论唑来膦酸法可诱导HIV感染者及健康对照者Vδ2T细胞大量增殖，其效率显著高于rδTCR单抗诱导法。Objective To find out the more efficient induction method through investigating the expansion effieiencies of HIV-infeeted patients＇ Vdelta2 T cells induced by zoledronic acid （Zol） or gammadelta TCR monoelonal antibody （mAb）. Methods 38 healthy control subjects （HC group） and 65 HIV infected patients （HIV group） were enrolled in this research. Peripheral blood mononuclear cells （PBMCs） of individuals were stimulated by Zol or rδ TCR mAb respectively for 14 days at 2. 0 × 107 cells/ well, and then rδ T cells and Vδ2 subsets frequencies were measured by flow cytometry （FCM） on 0, 7 and 14 day. The absolute numbers of Vδ2 T cells were calculated and the Vδ2 T cell expansion efficieneies by these two methods were compared. Results The absolute numbers and frequencies of Vδ2 T cell of HIV groups were lower than those of HC groups significantly on 0 day. After 14 days, the frequencies of Vδ2 T cell of HIV group and H C group were（ 17.6 ＋ 19.8）% and（64.3 ＋ 4.5 ）% respectively, and the expansion indexes of Vδ2 T cell were 54 ± 40 and 74 ± 29 respectively by induction of rδ TCR mAb. However, the frequencies of Vδ2 T cell of HIV group and HC group were（69.6 ± 21.2）% and（97.3 ± 1.7）% respectively, and the Vδ2 T cell expansion indexes were 538 ± 11 and 5984 ±721 respectively by induction of Zol. Conclusion Zol could induce the vast expansion of Vδ2 T cells of HIV infected patients. The expansion efficiency by Zol was better than that by the rδ TCR mAb.

关 键 词：HIV T淋巴细胞 膦酸类 

分 类 号：R512.91[医药卫生—内科学]