骨折局部注射结缔组织生长因子后骨保护素和血管内皮生长因子的表达  

Expression of osteoprotegerin and vascular endothelial growth factor after local injection of connective tissue growth factor

作　　者：丁明聪[1] 魏攀登[2] 林清[2] 杜斌[2] 台会平[3] 陈志信[3] 

机构地区：[1]兰州大学第二医院急救中心创伤外科,甘肃省兰州市730030 [2]兰州大学第一临床学院,甘肃省兰州市730000 [3]甘肃省人民医院骨一科,甘肃省兰州市730000

出　　处：《中国组织工程研究》2012年第48期9050-9053,共4页Chinese Journal of Tissue Engineering Research

摘　　要：背景:研究发现结缔组织生长因子在软骨发育过程中起重要作用,并具有强烈的促血管增殖作用,但其对骨折愈合的影响尚不清楚。目的:观察局部注射结缔组织生长因子对骨折愈合过程中骨保护素和血管内皮生长因子表达的影响及作用机制。方法:40只SD大鼠随机分为2组,建立胫骨干骨折模型后实验组动物即刻在骨折断端注射结缔组织生长因子0.1μg/kg,对照组注射等量生理盐水,隔日1次。结果与结论:实验组骨痂组织的早期即可见大量软骨细胞;免疫组织化学染色显示实验组骨保护素和血管内皮生长因子的表达均较对照组明显增强(P<0.05),并且两者表达的高峰期与对照组相比有所延长,有效缩短了两者表达高峰期之间的时间间隔。提示局部注射外源性生长因子结缔组织生长因子能增加骨折愈合过程中骨保护素和血管内皮生长因子在骨痂组织中的表达,并能改变两者的表达高峰期进而促进骨折愈合。BACKGROUND：Studies have found that connective tissue growth factor plays an important role in the cartilage development process with a strong angiogenic proliferative effect,but its influence on fracture healing is not clear.OBJECTIVE：To explore the effects of connective tissue growth factor on the expression of osteoprotegerin and vascular endothelial growth factor during fracture healing and its mechanism.METHODS：Forty Sprague Dawley rats were randomly divided into two groups.After the tibial bone fracture model was established,the rats in the experimental group were injected with 0.1 μg/kg connective tissue growth factor at the sites of the bone fracture,rats in the control group were injected with the same volume of normal saline.This was carried once every 2 days.RESULTS AND CONCLUSION：The hematoxylin-eosin staining indicated that more chondrocytes were found at early-stage of callus in experimental group.The immunohistochemistry staining showed that the expression levels of osteoprotegerin and vascular endothelial growth factor in the experimental group were higher than those in the control group at each period（P 0.05）.Compared with the control group,expression peak of both osteoprotegerin and vascular endothelial growth factor have extended in the experimental group.This effectively shortened the time interval of both expression peak.Connective tissue growth factor can improve the expression levels of osteoprotegerin and vascular endothelial growth factor in callus tissue during fracture healing,and it can also promote the fracture healing by changing the expression peak of both osteoprotegerin and vascular endothelial growth factor.

关 键 词：结缔组织生长因子 骨折愈合 骨病 骨保护素 血管内皮生长因子 大鼠 组织工程 组织构建 

分 类 号：R318[医药卫生—生物医学工程]