甲状腺激素对氚致神经元迁移障碍的改善作用  

Effects of thyroxine on the migration of hippocampal neurons in newborn rat exposed to HTO

作　　者：蔡二朋[1] 邱俊[1] 王永生[1] 吴翠萍[1] 姚晓波[1] 王明明[1] 

机构地区：[1]安徽医科大学放射医学研究所,合肥230032

出　　处：《中华放射医学与防护杂志》2012年第6期588-592,共5页Chinese Journal of Radiological Medicine and Protection

基　　金：安徽省自然科学基金（1208085MH162）;安徽省教育厅自然科学基金（KJ20112162）;中科院新型薄膜太阳电池重点实验室开放基金（2010007）

摘　　要：目的探讨甲状腺激素对氚辐射所致大鼠海马神经元迁移障碍的改善作用。方法取新生大鼠进行海马神经元培养，培养7d后将细胞随机分为对照组、氚水组、甲状腺激素组和氚水＋甲状腺激素组（同时加入3．7×10^5Bq／ml氚水与0．3μg／ml甲状腺激素），作用24h后，用LeicaAF6000活细胞工作站测神经细胞迁移距离；RT—PCR法检测reelinmRNA、BDNFmRNA的变化；Westernblot、免疫细胞化学染色法观察神经细胞内β-微管蛋白变化。结果与对照组相比，氚水组ReelinmRNA、BDNFmRNA和β-微管蛋白的表达量均显著减少（t=5．80、5．48、5．47，P〈0．01）；而氚水＋甲状腺激素组、甲状腺激素组的表达量均显著增加（t=7．75、12．06、13．65，P〈0．01；t=4．34、5．47、5．65，P〈0．01），并明显高于氚水组（t=2．92、10．32、8．76，P〈0．01；t=18．07、20．55、40．13，P〈0．01）。氚水组细胞迁移距离明显缩短（t=8．62，P〈0．01）；而氚水＋甲状腺激素组、甲状腺激素组细胞迁移距离明显延长（t=7．64、4．93，P〈0．01），并明显大于氚水组（t=11．32、12．31，P〈0．01）。结论甲状腺激素对氚辐射造成的神经元迁移障碍具有改善作用。Objective To explore the effect of thyroxine （TH） on the migration of hippocampal neurons in newborn rat exposed to tritiated water （HTO）. Methods The hippocampal neurons from neonatal rats were primarily cultured, 7 days later, randomly divided into control group, HTO group, TH group and HTO ＋ TH group（3.7 × 10^6 Bq/ml HTO and 0. 3 μg/ml TH were simultaneously added）. After 24 h, the distance of neuronal migration was measured with Leica AF 6000, the expressions of BDNF and Reelin mRNA in neurons were analyzed with reverse transcription polymerase chain reaction （RT-PCR） , the expression of β-tubulin protein in neurons was assayed with Western blot and immunocytochemical staining. Results Compared with control group, the expression of Reelin mRNA, BDNF mRNA and β- tubulin in HTO group were significantly reduced（ t = 5.80,5.48,5.47 ,P 〈 0.01 ） , but those in HTO ＋ TH group and TH group were obviously increased （ t = 7. 75,12. 06,13.65, P 〈 0. 01 ; t =- 4. 34,5.47,5.65,P 〈 0. 01 ） and higher than that in HTO group （ t = 2.92,10.32,8.76, P 〈 0. 01 ; t = 18.07,20.55,40. 13, P 〈 0. 01）. Accordingly, the neuronal migration distance in HTO group was much shorter than that in control （t = 8.62,P 〈 0. 01 ） , and in HTO ＋ TH group and TH group was far longer than that in control（t = 7. 64, 4. 93 ,P 〈 0. 01 ）. Moreover, the neuronal migration distance in HTO ＋ TH group was notably elongated in comparison with that in HTO group （ t = 11.32,12. 31, P 〈 0.01 ）. Conclusions Thyroxine may promote the migration of hippocampal neurons in newborn rat exposed to HTO.

关 键 词：甲状腺激素 氚辐射 神经元迁移 Β-微管蛋白 脑源性神经营养因子 

分 类 号：R96[医药卫生—药理学]