病理性瘢痕中胰岛素样生长因子1及受体mRNA的表达  

mRNA expression of insulin-like growth factor-1 and its receptor in pathological scars

作　　者：蔡浩[1,2] 唐冰[1] 朱家源[1] 朱斌[1] 毕良宽[1] 

机构地区：[1]中山大学附属第一医院烧伤外科,广东省广州市510080 [2]广东省惠东县人民医院外三科,广东省惠东县516300

出　　处：《中国组织工程研究》2012年第46期8629-8635,共7页Chinese Journal of Tissue Engineering Research

基　　金：广东省自然科学基金(8151008901000082);课题名称:人胰岛素样生长因子1基因修饰内皮祖细胞对糖尿病足溃疡的修复作用~~

摘　　要：背景:多种肿瘤中胰岛素样生长因子1受体及其配体表达异常,且胰岛素样生长因子1是多种细胞的有丝分裂原和抗细胞凋亡的因子,控制多种细胞的增殖、分化和凋亡的进程。目的:观察胰岛素样生长因子1及胰岛素样生长因子1受体在病理性瘢痕中的表达。方法:收集中山大学附属第一医院烧伤外科收治患者增生性瘢痕、瘢痕疙瘩及其正常皮肤等手术切除标本,荧光定量实时PCR检测不同组织中胰岛素样生长因子1及其受体mRNA的表达水平。结果与结论:瘢痕疙瘩及增生性瘢痕中胰岛素样生长因子1mRNA表达水平均高于正常皮肤(P<0.05),且在瘢痕疙瘩及增生性瘢痕组织中胰岛素样生长因子1表达水平差异无显著性意义(P>0.05),胰岛素样生长因子1受体在增生性瘢痕表达水平与正常皮肤接近(P>0.05);而瘢痕疙瘩中胰岛素样生长因子1受体明显高于瘢痕周围正常皮肤(P<0.05)。说明胰岛素样生长因子1及其受体在病理性瘢痕的形成过程中起重要作用。BACKGROUND：In many tumors,insulin-like growth factor 1 and its receptor express abnormally.Insulin-like growth factor is the mitogen and anti-apoptotic factor of a variety of cells,controlling the proliferation,differentiation and apoptosis of various cells.OBJECTIVE：To observe the expression of insulin-like growth factor-1 and insulin-like growth factor-1 receptor in pathological scar.METHODS：Surgical specimens of hypertrophic scars,keloids and normal skin were collected from the patients admitted in the Department of Burn Surgery,First Affiliated Hospital of Sun Yat-sen University.Fluorescent quantitative real-time PCR technique was used to detect the mRNA expression of insulin-like growth factor-1 and insulin-like growth factor-1 receptor in different tissues.RESULTS AND CONCLUSION：The mRNA expression of insulin-like growth factor-1 was higher in the hypertrophic scars and keloids than in the normal skin（P0.05）.However,there was no difference in the mRNA expression of insulin-like growth factor-1 between the hypertrophic scars and keloids（P0.05）.The expression of insulin-like growth factor-1 receptor in the hypertrophic scars was close to that in the normal skin（P0.05）;while,the expression of insulin-like growth factor-1 receptor in the keloids was significantly higher than that in the surrounding tissues of keloids（P0.05）.These findings indicate that insulin-like growth factor-1 and its receptor play an important role in the formation of pathological scars.

关 键 词：瘢痕疙瘩 增生性瘢痕 胰岛素样生长因子1受体 胰岛素样生长因子1 定时荧光定量PCR 组织构建 组织工程 

分 类 号：R318[医药卫生—生物医学工程]