脱钙骨基质与骨髓间充质干细胞共培养关节腔内的成软骨活性  

Intra-articular chondrogenic activity of bone marrow mesenchymal stem cells co-cultured with allogenic demineralized bone matrix

作　　者：冯万文[1] 杨广智[1] 尹文化 柴玲霞 王晓红[1] 常伶文[1] 郭侃锁[1] 李婷[1] 沈逸萍 

机构地区：[1]徐州医学院附属连云港市东方医院骨科,江苏省连云港市222042 [2]东营市东城社区卫生服务中心,山东省东营市257091 [3]定西市人民医院,甘肃省定西市730500 [4]连云港市药物检验所,江苏省连云港市222000

出　　处：《中国组织工程研究》2012年第47期8741-8746,共6页Chinese Journal of Tissue Engineering Research

基　　金：江苏省连云港市卫生局资助项目(09014)~~

摘　　要：背景:将骨髓间充质干细胞附着到支架材料上再植入关节软骨缺损处,细胞不但不消失,而且可形成新的软骨。目的:观察同种异体脱钙骨基质与骨髓间充质干细胞共培养在关节内的成软骨活性。方法:在54只青紫蓝兔单侧膝关节制作关节软骨全层缺损模型,随机分组:实验组在缺损处植入自体骨髓间充质干细胞与同种异体脱钙骨基质复合物,对照组缺损处仅植入同种异体脱钙骨基质,空白对照组未植入任何物质。结果与结论:植入后12周,实验组缺损处修复组织呈软骨样,表面光滑平坦,与周围软骨整合的软骨细胞更为成熟,修复组织与软骨下骨结合牢固;修复组织的细胞为透明软骨样细胞,柱状排列,Ⅱ型胶原染色阳性,与周围软骨及软骨下骨整合良好,且实验组组织学评分优于对照组和空白对照组(P<0.01)。对照组缺损处修复组织呈纤维样,与周围软骨未结合,空白对照组缺损区无修复组织,两组均无Ⅱ型胶原染色阳性表达。表明同种异体脱钙骨基质与骨髓间充质干细胞共培养后植入膝关节可形成软骨样组织,有效修复关节软骨缺损。BACKGROUND： After embedded on scaffold materials and implanted into articular cartilage defects, bone marrow mesenchymal stem cells cannot disappear and may form new cartilages. To investigate intra-articular chondrogenic activity of bone marrow mesenchymal stem cells co-cultured with allogenic demineralized bone matrix.METHODS ： Models of full-thickness articular cartilage defects of the knee were prepared in 54 rabbits. The rabbits were randomly divided into three groups, Autelegous bone marrow mesenchymal stern cells-allogeneic demineralized bone matrix complex was implanted into articular cartilage defects in the knee of rabbits in the experimental group; allogeneic demineralized bone matrix was implanted in the control group; no intervention in blank control group. RESULTS AND CONCLUSION： At 12 weeks after transplantation, repaired tissues represented hyaline-like, smoothness and flat, the chondrocytes integrated with the surrounding cartilage were more mature, and the repaired tissues were integrated with subchondral bones firmly in the experimental group. Cells in the repaired tissues demonstrated hyaline cartilage-like and arranged columnedly, which were positive for typeⅡ collagen and integrated satisfactorily with native adjacent cartilages and subchondral bones. Histological scores of the experimental group were better than those of the control group and the blank control group （P 〈 0.01 ）. In the control group, the repaired tissues showed fibroid, and were not integrated with peripheral cartilages. In the blank control group, the repaired tissues were not observed. There was no positive type Ⅱ collagen expressed in both control groups. Those outcomes indicate cartilage-like tissues can form and articular cartilage defects can be repaired effectively after bone marrow mesenchymal stem cells co-cultured with allogeneic demineralized bone matrix were implanted into the knee joints.

关 键 词：脱钙骨基质 骨髓间充质干细胞 组织工程 关节软骨全层缺损 体内 共培养 关节软骨 修复 膝关节 

分 类 号：R318[医药卫生—生物医学工程]