Ag-TiO_2抗菌树脂基托的生物毒性评价  

Biological toxicity of resin base containing Ag-TiO_2 antibacterial agent

作　　者：张成伟[2] 刘杰[1] 葛亚丽[2] 王晗[2] 马术明[1] 

机构地区：[1]佳木斯大学附属口腔医院修复特诊,黑龙江省佳木斯市154002 [2]佳木斯大学口腔医学院,黑龙江省佳木斯市154002

出　　处：《中国组织工程研究》2012年第47期8779-8782,共4页Chinese Journal of Tissue Engineering Research

基　　金：黑龙江省研究生创新科研项目(YJSCX2011-388HLJ)~~

摘　　要：背景:纳米Ag-TiO2抗菌剂结合金属Ag+与纳米TiO2的抗菌性能,具有抗菌广谱、长久性、耐高温、安全、经济等特点。目的:评价Ag-TiO2抗菌树脂基托对人体的生物安全性。方法:①口腔黏膜刺激实验:将含0(对照),0.5%,1.0%,1.5%,2.0%,3.0%纳米Ag-TiO2抗菌剂的牙托粉缝合于成年金黄地鼠颊黏膜中14d。②体外细胞毒性实验:将小鼠成纤维细胞L-929接种于含0(对照),0.5%,1.0%,1.5%,2.0%,3.0%纳米Ag-TiO2抗菌剂的牙托粉浸提液中培养。结果与结论:①黏膜刺激实验显示,与不同浓度抗菌树脂基托接触的颊黏膜与对照组切片无差异,未出现过敏或炎症反应。②细胞毒性实验证实,各浓度Ag-TiO2抗菌树脂基托无细胞毒性,毒性反应为0级。说明Ag-TiO2抗菌树脂基托符合中华人民共和国国家标准及相关的医药行业标准,对人体安全、无害,具有良好的生物相容性。BACKGROUND： Nano Ag-TiO2 antibacterial agent is combined with the antibacterial properties of Ag＋ and nano Tie2 and widely used in various antibacterial products for its characteristics of broad-spectrum antibiotic, long-lasting effect, high temperature resistance, safety, and economy. OBJECTIVE： To evaluate the bio-security of resin base containing Ag-TiO2 antibacterial agent on human body. METHODS： First, oral mucosa irritation test： denture powder containing 0（control）, 0.5%,1%,1.5%,2% and 3% Ag-TiO2 antibacterial agents was sutured on the two sides of buccal mucosa of adult golden hamster for 14 days. Then, in vitro toxicity test： Mouse fibroblasts 1-929 were seeded on the leaching solution of denture powder containing 0 （control）, 0.5%, 1%, 1.5%, 2% and 3% Ag-TiO2 antibacterial agents to culture. RESULTS AND CONCLUSION： ①Mucous membrane irritating test showed that there was no difference between buccal mucosa contacted with different concentrations of antibacterial resin base and control group of slices. Besides, no allergy or inflammation was found. ②Cytotoxicity test confirmed that various concentrations of Ag-TiO2 antibacteria resin base showed no cytotoxicity, and toxic reaction to level 0. These results suggest that Ag-TiO2 antibacterial resin base conforms to the People＇s Republic of China national and relevant pharmaceutical industry standards. It is secure and harmless to human body, moreover, it has good biocompatibility.

关 键 词：Ag-TiO2抗菌剂 树脂基托 生物相容性 细胞毒性 

分 类 号：R318[医药卫生—生物医学工程]