猪细小病毒CG-05株在ST细胞上的增殖规律研究  

Replication Dynamics of Porcine Parvovirus CG-05 Strain in ST Cell Culture

作　　者：唐满华[1,2] 陈瑞爱[1,2] 何玲[1] 梁桂益 

机构地区：[1]广东大华农动物保健品股份有限公司,广东新兴527400 [2]肇庆大华农生物药品有限公司.广东省兽用生物制品生物技术研究与应用企业重点实验室,广东肇庆526238

出　　处：《中国兽药杂志》2013年第1期14-16,22,共4页Chinese Journal of Veterinary Drug

摘　　要：通过将猪细小病毒CG-05株同步接种于ST细胞,测定不同时间收获的病毒液的TCID50和HA,研究了CG-05株病毒在ST细胞上的增殖规律。病毒接种后镜检观察接毒细胞,在接种病毒后24 h,即可在细胞较少的区域看到非常轻微的病变。测定病毒TCID50,检测到接毒后培养17h病毒已经明显增殖;当培养到40h左右,其TCID50即接近高峰,达到10-7.2/0.1mL以上,并进入平台期;继续培养,TCID50最高可达到10-8.5/0.1mL,且直到122 h也不见明显降低。病毒HA价也出现同样的平台期,但比TCID50的平台期出现得晚,到50 h才进入平台期。结果表明,用ST细胞培养CG-05株病毒,接种病毒培养56～96 h后,如病变达到80%以上,且细胞脱落已形成20%以上空斑,此时收获病毒可获得高效价的病毒液。该研究可为制备高效价的PPV病毒抗原提供数据资料。The growth property of porcine parvovirus CG -05 strain in ST cells was studied by TCIDs0 and HA test. The results indicated that slight CPE could be observed and the TCIDs0 also rose obviously in 17 h after the virus be added into the cell culture. The TCID50 was near to the peak and soon reached a plateau phase （above 1072/0.1 mL） after about 40 h of culture. The TCID50 could reach 108＇5/0.1 mL by the most with no obvious dropping until 122 h. HA titer also rose with the culture time and soon reached a plateau phase in 50 h after the virus be added into the cell culture. According to the results, using ST ceils to propagate porcine parvovirus CG -05 strain, high TCIDs0 and HA titer virus could be gotten in 56 to 96 h after inoculation, when above 80% ceils of the culture appeared CPE and 20% of which detached from the bottle wall and appeared plaque. The research could give data support for the preparation of high titer PPV antigen.

关 键 词：猪细小病毒 ST细胞 增殖规律 

分 类 号：S852.659.2[农业科学—基础兽医学]