两种外科手术对2型糖尿病大鼠胰腺SUR1表达的影响  

Effect of Roux-en-Y Gastric Bypass and Biliopancreatic Diversion on Expression of SUR1 in Rats with Type 2 Diabetes Mellitus

作　　者：陈嘉[1] 王萧[1] 张永斌[1] 高欣[1] 陈苑[1] 董浩然[1] 曹崇波[1] 

机构地区：[1]广州中医药大学实验动物中心,广东广州510405

出　　处：《动物医学进展》2013年第1期24-28,共5页Progress In Veterinary Medicine

基　　金：广东省科技计划项目(2010B060500013);广东省科技计划项目(2011B060300007)

摘　　要：观察Roux-en-Y胃转流术(RYGBP)及胆胰分流术(BPD)对2型糖尿病(Type 2diabetes melli-tus,T2DM)大鼠胰腺组织磺脲类药物受体1(SUR1)的影响,探讨其治疗机制。喂饲高糖高脂饲料4周后,用链脲佐菌素(STZ)30 mg/kg一次性腹腔注射,建立T2DM大鼠模型。将成模T2DM大鼠随机分为RYGBP组(G组)20只,BPD组(B组)20只,假手术组(S组)10只,对照组(C组)10只。检测术前、术后1、2、3、4、8、16周空腹体重、血清葡萄糖(BG);检测术后16周胰腺SUR1mRNA和蛋白的表达。RYGBP、BPD组大鼠术后16周,空腹体重分别为338.9g±17.5g、333.3g±28.4g,BG值分别为9.7mmol/L±0.8mmol/L、11.9mmol/L±2.4mmol/L,与术前比较均明显降低(P<0.01)。RYGBP、BPD组SUR1mRNA和蛋白表达高于对照组(P<0.01),手术对SUR1的表达表现了上调作用。两个手术组手术时间、体重、血糖、SUR1的表达差异无显著性(P>0.05)。RYGBP组死亡率为20%,BPD组死亡率为35%。Roux-en-Y胃转流术和胆胰分流术治疗T2DM大鼠疗效相近,但胆胰分流术死亡率高于胃转流术。SUR1蛋白表达的降低可能是糖尿病发病的分子机制之一,手术治疗后表达加强,可能是手术治疗2型糖尿病的作用机制之一。To clarify effect of Roux-en-Y gastric bypass（RYGBP）and biliopancreatic diversion（BPD）on ex- pression of pancreatic sulfonylurea receptorl （SUR1） in rats with type 2 diabetes mellitus（T2DM）induced by streptozotocin（STZ）injection combination and high-sugar and high-fat diets. 70 rats with STZ and high- sugar and high-fat diets induced diabetes were randomly allocated into RYGBP group （G group, n = 20）, BPD group（B group,n=20）,sham operation group（S group,n=10）,control group（C group,n= 10）. The fasting blood glucose,the weight were measured before and after operation on 1st, 2nd,3rd,4th,8th and 16th week postoperation. The expression of protein and mRNA of SUR1 were measured after operation on 16th week. The fasting blood glucose of the G group descended to 9.7 mmol/L±0.8 mmol/L,the weight was 338.9 g±17.5 g. The fasting blood glucose of the B group descended to 11.9 mmol/L±2.4 mmool/L, the weight was 333.3 g±28.4 g. There were significant differences between values before and after opera- tionon 16th week（P〈0.01）. The expression of SUR1 in G group and B group were significantly higher than that in C group（P〈0.01）. The fasting blood glucose,the weight,the operation time had no signifi- cant differences between group G and B. The mortality was 20% in the G group and 35% in the 13 group. RYGBP and BPD have similar efficacies in treatment of rats with T2DM,and BPD can lead to higher mor- talities than RYGBP. The diminished expression of $URI in pancreatic tissue could be a molecular mecha- nism of diabetes,RYGBP and BPD can attenuate this change in some extent.

关 键 词：2型糖尿病 胃转流术 胆胰分流术 磺脲类药物受体1 

分 类 号：R587.1[医药卫生—内分泌] S857.12[医药卫生—内科学]