吸入性麻醉药对乳腺和大脑肿瘤细胞基因表达的调控  

作　　者：鞠志海 刘晓(译) 郭曲练(校) 

出　　处：《麻醉与镇痛》2012年第6期17-22,共6页Anesthesia & Analgesia

摘　　要：在临床实践中，基因表达技术已广泛应用于疾病的诊断、预后和治疗。我们假设吸入性麻醉药（volatileanesthetics，VA）能够影响肿瘤细胞的基因表达。在体外条件下，分别将神经元细胞系的SH-SY5Y细胞和乳腺细胞系的MCF7细胞暴露于各种吸入性麻醉药，如恩氟烷、异氟烷、地氟烷、氟烷、七氟烷或氧化亚氮（N2O），然后对所得的微阵列基因表达谱进行分析研究。我们发现，不同的挥发性麻醉药对不同水平的细胞培养基和不同时间点的基因表达有显著影响。吸入性麻醉药对一些用于预测乳腺癌遗传特征的基因也能产生影响。本研究提示VA以一种独特的、时间依赖的方式调节乳腺和大脑肿瘤培养细胞的基因表达。Gene expression is increasingly used for diagnostic, prognostic, and therapeutic purposes in dinical practice. We tested the hypothesis that volatile anesthetics （VA） affect gene expression of tumor cells. Cells from the neuronal cell line SH-SYSY and from the breast cell line MCF-7 were exposed ex vivo to enflurane, isofiurane, desflurane, halothane, sevoflurane, or nitrous oxide. Microarray gene expression profiles were studied. We observed significant differences in gene expression levels of cell cultures and response in time when exposed to different VA. Some genes used for predictive genetic fingerprints for breast cancer were affected by VA. Our findings suggest that VA modulate gene expression in breast and brain tumor cell cultures in a unique and time-dependent manner.

关 键 词：吸入性麻醉药 基因表达谱 肿瘤细胞 大脑肿瘤 乳腺癌 SH-SY5Y细胞 调控 人性 

分 类 号：R971.2[医药卫生—药品]